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- W322553559 abstract "A proportion of human lymphocytes form spontaneous rosettes with sheep red blood cells (SRBC). These rosette forming cells (RFC) respond well to phytohaemagglutinin (PHA) and are poor stimulators in mixed lymphocyte reactions (MLR). Ficoll-Triosil density gradients to separate RFC from non-RFC confirmed these findings. However, some stimulatory activity is regularly found in separated RFC. This could be because of (1) uniform weak activity of all RFC, (2) a subpopulation of RFC, (3) incomplete separation of RFC from non-RFC, or (4) “back stimulation” of stimulator by responder cells. This chapter describes an experiment to distinguish between these possibilities by using another method of separation—velocity sedimentation. In the experiment, three populations of cells were separated at room temperature by this method—RFC, non-RFC, and fragile RFC. From this experiment, it was found that RFC-enriched fractions and non-RFC fractions gave less good PHA responses than fragile RFC; these cells gave better PHA responses than unseparated cells. In MLR, RFC enriched fractions were poor stimulators despite the presence of monocytes in the responder population." @default.
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- W322553559 date "1976-01-01" @default.
- W322553559 modified "2023-09-27" @default.
- W322553559 title "Human lymphocyte subpopulations" @default.
- W322553559 doi "https://doi.org/10.1016/b978-0-12-233750-5.50050-6" @default.
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