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• W32290164 abstract "Received: Revised: Accepted: June 04, 2013 December 23, 2013 January 25, 2014 The study was performed to investigate the immunosuppressive effect of aflatoxin on the pathogenesis of H9N2 AI virus in SPF chickens. The experiment was carried out on 110 unvaccinated day old SPF chicks. They were divided into four groups of 25 birds each. Group I was kept as a non-treated and non-infected control; group II was intranasally infected with H9N2 AI virus at the 4 week of age; group III was fed on a diet containing 0.75 ppm aflatoxin from day one through the entire experiment period and group IV was fed on diet containing 0.75 ppm aflatoxin as group III and infected intranasally with H9N2 AI virus at the 4 week of age. Five chicks were kept as contact control (without infection) to group II and group IV. Five chickens from each group were slaughtered at 4, 9, 14, 20 and 27 DPI Serum was collected from all slaughtered birds (5 serum samples/group/slaughter time) for serology (HI). Specimens from nasal conchae, trachea, lungs, liver, kidneys, bursa of Fabricius, thymus, spleen, pancreas and brain were collected from slaughtered birds for histopathology and immunohistochemistry. The histopathological lesions were more severe and persist till the end of the experiment in group IV. Using immunoperoxidase technique viral antigens were detected in the nasal conchae, trachea, lungs, thymus, kidneys and brain in group II while in group IV it extended further to the pancreas and bursa of Fabricius. In conclusion, the immunosuppression caused by aflatoxin increased the severity of lesions and allowed the virus to be disseminated to more organs. ©2014 PVJ. All rights reserved" @default.
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• W32290164 date "2014-01-01" @default.
• W32290164 modified "2023-09-27" @default.
• W32290164 title "Effect of aflatoxin induced immunosuppression on pathogenesis of H9N2 avian influenza virus." @default.
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