Matches in SemOpenAlex for { <https://semopenalex.org/work/W324299> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W324299 startingPage "62" @default.
- W324299 abstract "Delta-aminolevulinic acid (ALA) is the universal precursor for the synthesis of chlorophylls and tetrapyrroles. The first enzyme of the pathway activates glutamate by ligating it to its cognate tRNA. The second enzyme is a dehydrogenase which converts the activated glutamate on glu-tRNA to glutamate-1-semialdehyde (GSA). We now report the purification of the second enzyme, GSA dehydrogenase by using ammonium sulfate fractionation and column chromatography with DEAE Sepharose, Blue Sepharose and 2{prime},5{prime}-ADP Agrose. The native molecular weight of this enzyme, as determined by HPLC, was 130 KD. We have developed a specific assay for the dehydrogenase by measuring the formation of labeled GSA from ({sup 3}H)-glu-tRNA. The efficiency of glu-tRNA to GSA conversion by the dehydrogenase was low. However, this rate could be increased many fold by the addition of glu-tRNA synthetase, glutamate and ATP. We think under in vivo conditions, the first two enzymes of ALA biosynthesis may form a complex which operates much more efficiently." @default.
- W324299 created "2016-06-24" @default.
- W324299 creator A5070515269 @default.
- W324299 creator A5072168175 @default.
- W324299 date "1990-05-01" @default.
- W324299 modified "2023-09-24" @default.
- W324299 title "Purification of the second enzyme of chlorophyll biosynthesis from Chlamydomonas reinhardtii" @default.
- W324299 hasPublicationYear "1990" @default.
- W324299 type Work @default.
- W324299 sameAs 324299 @default.
- W324299 citedByCount "1" @default.
- W324299 crossrefType "journal-article" @default.
- W324299 hasAuthorship W324299A5070515269 @default.
- W324299 hasAuthorship W324299A5072168175 @default.
- W324299 hasConcept C104317684 @default.
- W324299 hasConcept C143065580 @default.
- W324299 hasConcept C153957851 @default.
- W324299 hasConcept C170493617 @default.
- W324299 hasConcept C181199279 @default.
- W324299 hasConcept C185592680 @default.
- W324299 hasConcept C2776317432 @default.
- W324299 hasConcept C2777202666 @default.
- W324299 hasConcept C2777727519 @default.
- W324299 hasConcept C2779881004 @default.
- W324299 hasConcept C553450214 @default.
- W324299 hasConcept C55493867 @default.
- W324299 hasConcept C61174792 @default.
- W324299 hasConcept C67705224 @default.
- W324299 hasConcept C86803240 @default.
- W324299 hasConceptScore W324299C104317684 @default.
- W324299 hasConceptScore W324299C143065580 @default.
- W324299 hasConceptScore W324299C153957851 @default.
- W324299 hasConceptScore W324299C170493617 @default.
- W324299 hasConceptScore W324299C181199279 @default.
- W324299 hasConceptScore W324299C185592680 @default.
- W324299 hasConceptScore W324299C2776317432 @default.
- W324299 hasConceptScore W324299C2777202666 @default.
- W324299 hasConceptScore W324299C2777727519 @default.
- W324299 hasConceptScore W324299C2779881004 @default.
- W324299 hasConceptScore W324299C553450214 @default.
- W324299 hasConceptScore W324299C55493867 @default.
- W324299 hasConceptScore W324299C61174792 @default.
- W324299 hasConceptScore W324299C67705224 @default.
- W324299 hasConceptScore W324299C86803240 @default.
- W324299 hasLocation W3242991 @default.
- W324299 hasOpenAccess W324299 @default.
- W324299 hasPrimaryLocation W3242991 @default.
- W324299 hasRelatedWork W1132041150 @default.
- W324299 hasRelatedWork W1482607235 @default.
- W324299 hasRelatedWork W1491236279 @default.
- W324299 hasRelatedWork W1513467803 @default.
- W324299 hasRelatedWork W1536751808 @default.
- W324299 hasRelatedWork W1547657346 @default.
- W324299 hasRelatedWork W1547964373 @default.
- W324299 hasRelatedWork W1553140893 @default.
- W324299 hasRelatedWork W1580129210 @default.
- W324299 hasRelatedWork W1831182106 @default.
- W324299 hasRelatedWork W1966596561 @default.
- W324299 hasRelatedWork W1968532939 @default.
- W324299 hasRelatedWork W1975539417 @default.
- W324299 hasRelatedWork W2003676242 @default.
- W324299 hasRelatedWork W2022639937 @default.
- W324299 hasRelatedWork W2044998914 @default.
- W324299 hasRelatedWork W2055823541 @default.
- W324299 hasRelatedWork W2057424432 @default.
- W324299 hasRelatedWork W238379270 @default.
- W324299 hasRelatedWork W3026180836 @default.
- W324299 isParatext "false" @default.
- W324299 isRetracted "false" @default.
- W324299 magId "324299" @default.
- W324299 workType "article" @default.