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- W325661569 abstract "This chapter presents the double-layer modification that was developed to facilitate the detection of antibodies secreted by the progeny of clonable B cells. This allows a more complete analysis of B cell colonies, especially regarding questions of antigen responsiveness, repertoire, and isotype switch. It allows the unequivocal identification of B cells, which arise under conditions that are not themselves selective for B cell growth alone. It is also useful for preservation of colonies for subsequent morphological analysis and more recently is used to monitor RNA transcripts in developing colonies. Clonal assays based on limiting dilution liquid cultures, limiting dilution fragment cultures, and semisolid cultures are extensively utilized. Subsequent studies appeared that examined the characteristics of the B cells, which initiated colony formation based upon physical properties, organ distribution, cell surface phenotype, and other criteria." @default.
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- W325661569 date "1987-01-01" @default.
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- W325661569 title "Growth of B cell colonies in double-layer agar cultures" @default.
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- W325661569 doi "https://doi.org/10.1016/0076-6879(87)50083-0" @default.
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