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- W326550023 abstract "To explore flow cytometry (FCM) based method for automatic analysis of micronuclei (MN) in cells staining with anti-CD71-FITC and PI.Cell were fixed in -85 degrees C methanol and stained with anti-CD71-FITC and PI, instrument was optimized using malaria-infected erythrocytes as biological standards, a FCM based method for automatic analysis of micronuclei was established. In order to estimate the reliability of the method, the frequencies of micronucleated reticulocytes (MN-RET) in peripheral blood of NIH mice treated with colchicine (COL) was detected using the method.Four cell populations, normochromatic erythrocytes (NCE), reticulocytes (RET), micronucleated erythrocytes (MN-NCE) and MN-RET could be clearly resolved on the FL1-FL2 dot plot using this method. COL induced a dose-dependent increases in the frequency MN-RET in peripheral blood. Data obtained manually and automatically showed a strong linear correlation (r = 0.98) as far as the percentage of MN-RET.The FCM-based automatic technique using FITC-anti-CD71 and PI staining is a reliable tool for micronucleus analysis." @default.
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- W326550023 date "2005-01-01" @default.
- W326550023 modified "2023-09-28" @default.
- W326550023 title "[Automatic analysis of micronuclei by flow cytometry using anti-CD71-FITC and PI staining]." @default.
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