Matches in SemOpenAlex for { <https://semopenalex.org/work/W327291803> ?p ?o ?g. }
- W327291803 endingPage "1363" @default.
- W327291803 startingPage "1357" @default.
- W327291803 abstract "In order to test the potential of psoralen photoaddition for the probing of RNA conformation at sequence resolution, we have analyzed the specificity of the reaction of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) with Escherichia coli tRNAPhe. The sites of HMT covalent addition have been identified by a combination of analytical techniques involving chemical cleavage of the tRNAPhe molecule at the m7G site and gel electrophoresis of RNase T1 digests together with paper electrophoretic characterization of HMT-modified nucleotides and oligonucleotides. In addition, we have taken advantage of the alteration of the cleavage rate of pancreatic RNase adjacent to a photoadduct. HMT photoaddition shows a very high preference for uracil residues. However, important differences in HMT photoreactivity are observed for various U sites of the tRNAPhe molecule. Reactivity of specific bases has been correlated with partial melting of the molecule. Data available so far indicate a strong preference of the photoreactive probe for a loose helical conformation as compared with a tight helix, whereas a random coil appears poorly reactive." @default.
- W327291803 created "2016-06-24" @default.
- W327291803 creator A5040209154 @default.
- W327291803 creator A5062542148 @default.
- W327291803 date "1982-03-16" @default.
- W327291803 modified "2023-10-14" @default.
- W327291803 title "Specificity of the photoreaction of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen with RNA. Identification of reactive sites in E. coli tRNAPhe" @default.
- W327291803 cites W1966608511 @default.
- W327291803 cites W1970667469 @default.
- W327291803 cites W1971867701 @default.
- W327291803 cites W1978964400 @default.
- W327291803 cites W1979911590 @default.
- W327291803 cites W1979976739 @default.
- W327291803 cites W1981860248 @default.
- W327291803 cites W1985120622 @default.
- W327291803 cites W1987160917 @default.
- W327291803 cites W1988028055 @default.
- W327291803 cites W1994881877 @default.
- W327291803 cites W1997728919 @default.
- W327291803 cites W2009963879 @default.
- W327291803 cites W2011878394 @default.
- W327291803 cites W2012253277 @default.
- W327291803 cites W2024308598 @default.
- W327291803 cites W2027650483 @default.
- W327291803 cites W2030123313 @default.
- W327291803 cites W2035741483 @default.
- W327291803 cites W2036571617 @default.
- W327291803 cites W2037191152 @default.
- W327291803 cites W2040345052 @default.
- W327291803 cites W2057812123 @default.
- W327291803 cites W2068155744 @default.
- W327291803 cites W2068567113 @default.
- W327291803 cites W2086601588 @default.
- W327291803 cites W2178112091 @default.
- W327291803 cites W2415878564 @default.
- W327291803 doi "https://doi.org/10.1021/bi00535a039" @default.
- W327291803 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6176269" @default.
- W327291803 hasPublicationYear "1982" @default.
- W327291803 type Work @default.
- W327291803 sameAs 327291803 @default.
- W327291803 citedByCount "19" @default.
- W327291803 countsByYear W3272918032018 @default.
- W327291803 countsByYear W3272918032019 @default.
- W327291803 countsByYear W3272918032020 @default.
- W327291803 crossrefType "journal-article" @default.
- W327291803 hasAuthorship W327291803A5040209154 @default.
- W327291803 hasAuthorship W327291803A5062542148 @default.
- W327291803 hasConcept C104317684 @default.
- W327291803 hasConcept C10879258 @default.
- W327291803 hasConcept C129312508 @default.
- W327291803 hasConcept C151730666 @default.
- W327291803 hasConcept C153957851 @default.
- W327291803 hasConcept C175156509 @default.
- W327291803 hasConcept C178790620 @default.
- W327291803 hasConcept C185592680 @default.
- W327291803 hasConcept C32909587 @default.
- W327291803 hasConcept C43369102 @default.
- W327291803 hasConcept C512185932 @default.
- W327291803 hasConcept C552990157 @default.
- W327291803 hasConcept C55493867 @default.
- W327291803 hasConcept C67705224 @default.
- W327291803 hasConcept C69766542 @default.
- W327291803 hasConcept C71240020 @default.
- W327291803 hasConcept C86803240 @default.
- W327291803 hasConceptScore W327291803C104317684 @default.
- W327291803 hasConceptScore W327291803C10879258 @default.
- W327291803 hasConceptScore W327291803C129312508 @default.
- W327291803 hasConceptScore W327291803C151730666 @default.
- W327291803 hasConceptScore W327291803C153957851 @default.
- W327291803 hasConceptScore W327291803C175156509 @default.
- W327291803 hasConceptScore W327291803C178790620 @default.
- W327291803 hasConceptScore W327291803C185592680 @default.
- W327291803 hasConceptScore W327291803C32909587 @default.
- W327291803 hasConceptScore W327291803C43369102 @default.
- W327291803 hasConceptScore W327291803C512185932 @default.
- W327291803 hasConceptScore W327291803C552990157 @default.
- W327291803 hasConceptScore W327291803C55493867 @default.
- W327291803 hasConceptScore W327291803C67705224 @default.
- W327291803 hasConceptScore W327291803C69766542 @default.
- W327291803 hasConceptScore W327291803C71240020 @default.
- W327291803 hasConceptScore W327291803C86803240 @default.
- W327291803 hasIssue "6" @default.
- W327291803 hasLocation W3272918031 @default.
- W327291803 hasLocation W3272918032 @default.
- W327291803 hasOpenAccess W327291803 @default.
- W327291803 hasPrimaryLocation W3272918031 @default.
- W327291803 hasRelatedWork W1906502852 @default.
- W327291803 hasRelatedWork W1966773843 @default.
- W327291803 hasRelatedWork W1985875854 @default.
- W327291803 hasRelatedWork W2016147415 @default.
- W327291803 hasRelatedWork W2054281456 @default.
- W327291803 hasRelatedWork W2078865641 @default.
- W327291803 hasRelatedWork W2092722787 @default.
- W327291803 hasRelatedWork W2097625555 @default.
- W327291803 hasRelatedWork W2433397146 @default.
- W327291803 hasRelatedWork W2462923179 @default.
- W327291803 hasVolume "21" @default.
- W327291803 isParatext "false" @default.