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- W32902235 abstract "The presence of phosphoinositide-mobilizing enzymes has been investigated in purified rat liver nuclei by radiolabelling and by probing with antibodies. A Ca(2+)-activated phosphoinositidase C (PIC) is present and was shown immunologically to be the beta 1 isoform. No gamma- or delta-PIC was found. However, only 5% of the total beta 1-PIC isoform is nuclear, with the majority being cytosolic. G alpha q and G alpha 11, the suggested physiological activators of beta 1-PIC, were not present. A PtdIns4P 5-kinase is also present, which immunologically is shown to be the C isoform. All of these nuclear inositide enzymes still remained after the removal of the nuclear envelope with Triton X-100, consistent with the concept of an intranuclear inositide cycle [Divecha, Banfic and Irvine (1991) EMBO. J. 10, 3207-3214]." @default.
- W32902235 created "2016-06-24" @default.
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- W32902235 date "1993-02-01" @default.
- W32902235 modified "2023-10-16" @default.
- W32902235 title "Phosphoinositide signalling enzymes in rat liver nuclei: phosphoinositidase C isoform <i>β</i>1 is specifically, but not predominantly, located in the nucleus" @default.
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- W32902235 doi "https://doi.org/10.1042/bj2890617" @default.
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