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• W32953799 abstract "Temperature is arguably the leading factor that drives adaptation of organisms and ecosystems. Remarkably, many sister species share the same habitat because of their different temporal or micro-spatial thermal adaptation. In this PhD, the underlying molecular mechanisms of the adaptation of closely related species to different temperatures are sought. A thermodynamic analysis was applied to a genome-scale metabolic model of S. cerevisiae at warm and cold temperatures to identify thermo-dependent reactions. Gene Ontology (GO) analysis of predicted cold-dependent reactions found that redox reactions were significantly enriched. A complementary large scale experimental approach was taken by competing 6,000 mutant strains at 16�C to identify genes that were responsible for the fitness at low temperatures. The experiment was carried out in three different nutritional conditions to test the plasticity of temperature dependency. A list of strains whose copy number significantly increased or decreased in all media conditions was constructed and analysed using Gene Ontology. Vitamin biosynthesis, lipid/fatty acid processes and oxido-reduction reactions were all found to be significantly affected by the cold condition. Combining the data from the two studies a list of candidate genes affected by temperature changes were generated. In particular, two genes, GUT2 and ADH3, were identified as potential cold favouring genes and studied in more detailed. Mutants for these two genes were created in a pair of natural sympatric cryotolerant and thermotolerant Saccharomyces yeasts, namely S. kudriavzevii CA111 and S. cerevisiae 96.2, representing an excellent ecological experimental model for differential temperature adaption. My results showed that when compared to the parental strains, both mutants showed lower fitness at cold temperatures as predicted, and in S. kudriavzevii CA111 these mutations significantly improve growth at warm temperatures. Results from all aspects of this work indicate that oxidation reduction reactions are important for cold acclimation. It is known that heat stress causes redox imbalances which are compensated by increasing glycerol production or cytosolic acetaldehyde. Since GUT2 and ADH3 are involved in these processes, mutations in these genes may not be able to compensate for temperature changes. My data also shows that vitamins may also play an important role in cold acclimation which would be an interesting line of investigation for future work. Overall this PhD thesis has incorporated in silico and in vivo work to identify potential processes and genes involved in the temperature adaptation of sister Saccharomyces yeast species. The approach and results provided in this study support the use of a systems biology framework to studying species adaptation to environmental changes, and show that such models can yield testable predictions that may lead to new biological discoveries." @default.
• W32953799 created "2016-06-24" @default.
• W32953799 creator A5082331125 @default.
• W32953799 date "2013-03-03" @default.
• W32953799 modified "2023-09-27" @default.
• W32953799 title "Environmental systems biology of temperature adaptation in yeast" @default.
• W32953799 cites W1536226251 @default.
• W32953799 cites W1540148631 @default.
• W32953799 cites W1543388089 @default.
• W32953799 cites W1552459853 @default.
• W32953799 cites W1584117454 @default.
• W32953799 cites W1632341477 @default.
• W32953799 cites W1641544182 @default.
• W32953799 cites W1775602956 @default.
• W32953799 cites W1797657318 @default.
• W32953799 cites W1850569493 @default.
• W32953799 cites W1899785354 @default.
• W32953799 cites W1917144541 @default.
• W32953799 cites W1965876012 @default.
• W32953799 cites W1968913041 @default.
• W32953799 cites W1972740086 @default.
• W32953799 cites W1975597731 @default.
• W32953799 cites W1976841360 @default.
• W32953799 cites W1978143896 @default.
• W32953799 cites W1978408474 @default.
• W32953799 cites W1978486653 @default.
• W32953799 cites W1985687822 @default.
• W32953799 cites W1990647376 @default.
• W32953799 cites W1994134392 @default.
• W32953799 cites W1994624068 @default.
• W32953799 cites W1994683825 @default.
• W32953799 cites W1995808589 @default.
• W32953799 cites W1998767819 @default.
• W32953799 cites W2003099891 @default.
• W32953799 cites W2003835605 @default.
• W32953799 cites W2008875008 @default.
• W32953799 cites W2010595044 @default.
• W32953799 cites W2012479817 @default.
• W32953799 cites W2013314158 @default.
• W32953799 cites W2014146396 @default.
• W32953799 cites W2016786878 @default.
• W32953799 cites W2018902287 @default.
• W32953799 cites W2021955476 @default.
• W32953799 cites W2023805910 @default.
• W32953799 cites W2024725497 @default.
• W32953799 cites W2026089579 @default.
• W32953799 cites W2027126666 @default.
• W32953799 cites W2028762212 @default.
• W32953799 cites W2029021131 @default.
• W32953799 cites W2030776070 @default.
• W32953799 cites W2032824523 @default.
• W32953799 cites W2036099201 @default.
• W32953799 cites W2042067428 @default.
• W32953799 cites W2043417285 @default.
• W32953799 cites W2047632644 @default.
• W32953799 cites W2048214825 @default.
• W32953799 cites W2053184061 @default.
• W32953799 cites W2054624670 @default.
• W32953799 cites W2054719673 @default.
• W32953799 cites W2055172549 @default.
• W32953799 cites W2062819607 @default.
• W32953799 cites W2066837662 @default.
• W32953799 cites W2070437441 @default.
• W32953799 cites W2075309657 @default.
• W32953799 cites W2076505192 @default.
• W32953799 cites W2077724722 @default.
• W32953799 cites W2079647856 @default.
• W32953799 cites W2081775875 @default.
• W32953799 cites W2081933515 @default.
• W32953799 cites W2083011190 @default.
• W32953799 cites W2083527495 @default.
• W32953799 cites W2086074044 @default.
• W32953799 cites W2090803844 @default.
• W32953799 cites W2091888493 @default.
• W32953799 cites W2094780854 @default.
• W32953799 cites W2099735525 @default.
• W32953799 cites W2100057937 @default.
• W32953799 cites W2102164505 @default.
• W32953799 cites W2104183222 @default.
• W32953799 cites W2104775769 @default.
• W32953799 cites W2106104636 @default.
• W32953799 cites W2106132742 @default.
• W32953799 cites W2106576546 @default.
• W32953799 cites W2107409961 @default.
• W32953799 cites W2107508235 @default.
• W32953799 cites W2108703094 @default.
• W32953799 cites W2109657087 @default.
• W32953799 cites W2111905282 @default.
• W32953799 cites W2113030587 @default.
• W32953799 cites W2116398954 @default.
• W32953799 cites W2117720828 @default.
• W32953799 cites W2118655754 @default.
• W32953799 cites W2123782813 @default.
• W32953799 cites W2124985265 @default.
• W32953799 cites W2126582986 @default.
• W32953799 cites W2128572161 @default.
• W32953799 cites W2132757418 @default.
• W32953799 cites W2132919995 @default.
• W32953799 cites W2133465414 @default.
• W32953799 cites W2133657715 @default.

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