Matches in SemOpenAlex for { <https://semopenalex.org/work/W330158624> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W330158624 endingPage "54" @default.
- W330158624 startingPage "54" @default.
- W330158624 abstract "The application of 2DE to membrane proteins is hampered by the limited protein solubility. In addition, the gel matrix is a limiting factor in combination with downstream analytical techniques such as mass spectrometry. Free-Flow Electrophoresis (FFE) in the isoelectric focusing (IEF) mode provides a carrier-free alternative to 2DE. IEF-FFE has proven useful for soluble proteins since the methodology is not limited in pH or size range. Various detergents have been applied to handle more hydrophobic proteins under denaturing conditions. However, a prerequisite for IEF-FFE is the maintained solubility of the proteins at their pI. This is not the case for many membrane proteins which have high tendency to precipitate at their respective pI.Here we present Interval Zone FFE (IZ-FFE) as a novel mode of FFE which facilitates separation of charged molecular species including proteins and protein complexes. In contrast to IEF, the separation is carried out at a constant pH relying on the net charges of the proteins. The applied pH may be significantly different from the pI of the proteins to be separated thus maintaining proteins in solution that would otherwise precipitate. Resolution and solubility may be increased further under denaturing conditions using urea and/or thiourea and by including non-ionic or zwitterionic detergents.IZ-FFE was applied to a total protein extract from HeLa cells and compared to IEF-FFE. A significant increase in protein solubility was observed in the IZ-FFE mode. Separations were performed using detergent-free media at high loading rates without observing precipitation. This was in contrast to IEF-FFE where precipitation was observed even when detergents were included in the media. The detergent-free separation media facilitated the coupling to RPLC-MS/MS since no detergent removal was required and the proteins were maintained in solution." @default.
- W330158624 created "2016-06-24" @default.
- W330158624 creator A5004247926 @default.
- W330158624 creator A5015115315 @default.
- W330158624 creator A5049282616 @default.
- W330158624 creator A5086115176 @default.
- W330158624 creator A5088909146 @default.
- W330158624 date "2007-02-01" @default.
- W330158624 modified "2023-09-28" @default.
- W330158624 title "P155-M Keeping Proteins in Solution: Interval Free-Flow Zone Electrophoretic Separation of a Total Protein Extract from HeLa Cells" @default.
- W330158624 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2292014" @default.
- W330158624 hasPublicationYear "2007" @default.
- W330158624 type Work @default.
- W330158624 sameAs 330158624 @default.
- W330158624 citedByCount "0" @default.
- W330158624 crossrefType "journal-article" @default.
- W330158624 hasAuthorship W330158624A5004247926 @default.
- W330158624 hasAuthorship W330158624A5015115315 @default.
- W330158624 hasAuthorship W330158624A5049282616 @default.
- W330158624 hasAuthorship W330158624A5086115176 @default.
- W330158624 hasAuthorship W330158624A5088909146 @default.
- W330158624 hasConcept C101660505 @default.
- W330158624 hasConcept C107054158 @default.
- W330158624 hasConcept C121332964 @default.
- W330158624 hasConcept C144647389 @default.
- W330158624 hasConcept C153294291 @default.
- W330158624 hasConcept C155574463 @default.
- W330158624 hasConcept C178790620 @default.
- W330158624 hasConcept C181199279 @default.
- W330158624 hasConcept C185592680 @default.
- W330158624 hasConcept C23347341 @default.
- W330158624 hasConcept C35859425 @default.
- W330158624 hasConcept C40250595 @default.
- W330158624 hasConcept C41625074 @default.
- W330158624 hasConcept C43617362 @default.
- W330158624 hasConcept C50476208 @default.
- W330158624 hasConcept C55493867 @default.
- W330158624 hasConcept C8985478 @default.
- W330158624 hasConcept C96294017 @default.
- W330158624 hasConceptScore W330158624C101660505 @default.
- W330158624 hasConceptScore W330158624C107054158 @default.
- W330158624 hasConceptScore W330158624C121332964 @default.
- W330158624 hasConceptScore W330158624C144647389 @default.
- W330158624 hasConceptScore W330158624C153294291 @default.
- W330158624 hasConceptScore W330158624C155574463 @default.
- W330158624 hasConceptScore W330158624C178790620 @default.
- W330158624 hasConceptScore W330158624C181199279 @default.
- W330158624 hasConceptScore W330158624C185592680 @default.
- W330158624 hasConceptScore W330158624C23347341 @default.
- W330158624 hasConceptScore W330158624C35859425 @default.
- W330158624 hasConceptScore W330158624C40250595 @default.
- W330158624 hasConceptScore W330158624C41625074 @default.
- W330158624 hasConceptScore W330158624C43617362 @default.
- W330158624 hasConceptScore W330158624C50476208 @default.
- W330158624 hasConceptScore W330158624C55493867 @default.
- W330158624 hasConceptScore W330158624C8985478 @default.
- W330158624 hasConceptScore W330158624C96294017 @default.
- W330158624 hasIssue "1" @default.
- W330158624 hasLocation W3301586241 @default.
- W330158624 hasOpenAccess W330158624 @default.
- W330158624 hasPrimaryLocation W3301586241 @default.
- W330158624 hasRelatedWork W1963948295 @default.
- W330158624 hasRelatedWork W1976523086 @default.
- W330158624 hasRelatedWork W1976590797 @default.
- W330158624 hasRelatedWork W1983259877 @default.
- W330158624 hasRelatedWork W1987767012 @default.
- W330158624 hasRelatedWork W1997081866 @default.
- W330158624 hasRelatedWork W2014774127 @default.
- W330158624 hasRelatedWork W2039147395 @default.
- W330158624 hasRelatedWork W2052308351 @default.
- W330158624 hasRelatedWork W2075818688 @default.
- W330158624 hasRelatedWork W2077912973 @default.
- W330158624 hasRelatedWork W2125582000 @default.
- W330158624 hasRelatedWork W2134852489 @default.
- W330158624 hasRelatedWork W2149660167 @default.
- W330158624 hasRelatedWork W2353530743 @default.
- W330158624 hasRelatedWork W2606765462 @default.
- W330158624 hasRelatedWork W2614158777 @default.
- W330158624 hasRelatedWork W88458564 @default.
- W330158624 hasRelatedWork W2489206404 @default.
- W330158624 hasRelatedWork W3139908524 @default.
- W330158624 hasVolume "18" @default.
- W330158624 isParatext "false" @default.
- W330158624 isRetracted "false" @default.
- W330158624 magId "330158624" @default.
- W330158624 workType "article" @default.