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- W330420312 abstract "The identification of the genetic purity of F1 hybrid seeds in new variety, rape “Zayou No59” was made via poly morphic RAPD (Random Amplified polymorphic DNA) markers. Using 40 ten mer arbitrary oligonucheotide primers the parents of “Zayou No. 59” were analyzed will RAPD. Approximately 290 products, ranging from 220～3530 base pairs(bp), were amplified. The majority of the products were identical in both “Ken C8” and “Shan 3A”. Primer Opa 06, Opc 12, Opc 20, Opk 03, Opk 13, Opj 12 generated polymorphic products. It was found that the reproducibility of primer Opk 03 was steady after repeating the tests. It could produce 5 bright bands, the fragment at 510 bp unique to the female line, and the fragment at 260 bp unique to the male line. The two fragments were complementary and stable in the F1 generation.The sampling selection of DNA sample used in identification of the purity in F1 was discussed in this report. Fig 3, Ref 11" @default.
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- W330420312 date "2000-01-29" @default.
- W330420312 modified "2023-09-25" @default.
- W330420312 title "IDENTIFICATION OF GENETIC PURITY OF SEED OF HYBRID RAPE “ZAYOU No.59” WITH RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)" @default.
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