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• W330733493 abstract "This paper presents an efficient protocol for rapid in vitro propagation of Stevia rebaudiana (Bert.) by nodal explants collected from mature plants placed on MS medium containing 1.0 mg l 6-benzylamino purine (BAP) or indole-3-butyric acid (IBA) either alone or in combination (1 mg l BAP with 0.5 mg l IBA) for shoot bud initiation. In order to induce multiple shoots, in vitro derived shoot buds from nodal explants were cultured on MS medium supplemented with different concentrations of IBA or indole-3-acetic acid (IAA; 0, 0.25, 0.5 mg l) in combination with BAP (1 mg l). Shoot buds were also placed on MS medium fortified only with IBA (0.5 mg l). Elongated shoots (3 cm) were transferred onto the rooting medium containing MS salts, vitamins and different concentrations of IBA or IAA (0, 0.25, 0.5 mg l) used individually for root induction. Results showed that the highest percent response (92.10%) was obtained on MS medium supplemented with BAP (1 mg l) and IBA (0.5 mg l) and hence this combination was found to be the most effective for shoot bud initiation. In addition, BAP (1 mg l) and IAA (0.25 mg l) combination was superior for multiple shoot bud induction (4.25 shoots). However, when shoot buds were placed on MS medium fortified only with 0.5 mg l IBA, few multiple shoots (1.37 shoots/explant) were induced. In fact, shoots produced roots within two weeks of culture; the highest percent (97%) of root induction and the maximum number of roots (8.90 roots/shoot) as well as the root length (1.70 cm) were observed on MS medium fortified with IAA (0.50 mg l). Rooted plantlets were successfully acclimatized in pots containing peat. Thus, this study provides an efficient and reproducible in vitro propagation protocol for rapid multiplication of Stevia rebaudiana and hence could be helpful to establish and cultivate this medicinal species as a promising newly introduced non caloric sweetener and antidiabetic medicinal plant in Tunisia." @default.
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• W330733493 date "2012-01-01" @default.
• W330733493 modified "2023-09-24" @default.
• W330733493 title "In vitro propagation of Stevia rebaudiana (Bert.) - a non caloric sweetener and antidiabetic medicinal plant." @default.
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