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• W33138032 abstract "Research Article1 March 1993free access A chimeric toxin to study the role of the 21 kDa GTP binding protein rho in the control of actin microfilament assembly. P. Aullo P. Aullo Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. Search for more papers by this author M. Giry M. Giry Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. Search for more papers by this author S. Olsnes S. Olsnes Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. Search for more papers by this author M.R. Popoff M.R. Popoff Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. Search for more papers by this author C. Kocks C. Kocks Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. Search for more papers by this author P. Boquet P. Boquet Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. Search for more papers by this author P. Aullo P. Aullo Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. Search for more papers by this author M. Giry M. Giry Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. Search for more papers by this author S. Olsnes S. Olsnes Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. Search for more papers by this author M.R. Popoff M.R. Popoff Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. Search for more papers by this author C. Kocks C. Kocks Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. Search for more papers by this author P. Boquet P. Boquet Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. Search for more papers by this author Author Information P. Aullo1, M. Giry1, S. Olsnes1, M.R. Popoff1, C. Kocks1 and P. Boquet1 1Unité des Toxines Microbiennes URA CNRS 557, Institut Pasteur, Paris, France. The EMBO Journal (1993)12:921-931https://doi.org/10.1002/j.1460-2075.1993.tb05733.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info We have developed a new tool for studying the role of rho in actin stress fibre formation. Clostridium botulinum exoenzyme C3 which affects actin microfilament assembly by ADP-ribosylation of p21 rho was genetically fused in various ways to diphtheria toxin (DT). The resulting chimeric toxins were tested on Vero cells. Chimeras of C3 and both the A and B fragments of diphtheria toxin had reduced cell binding activities but were apparently able to penetrate into Vero cells by the same mechanism as DT. Upon exposure to low pH, DC3B, a fusion protein of C3 and DT B fragment, had a high affinity for the DT receptor, but was apparently not able to translocate to the cytosol upon acidification. In spite of this, addition of picomolar concentrations of DC3B to the growth medium caused disruption of the cell microfilament system associated with vinculin and blocked cell growth efficiently, indicating that the C3 part of DC3B reached the cytosol, albeit by a different mechanism than that of whole diphtheria toxin. The chimeric DC3B toxin was also applied to Vero cells infected by Listeria monocytogenes, a pathogenic bacterium that uses an unknown mechanism of actin polymerization to move rapidly in the cytosol. DC3B inhibited the bacterially induced microfilament assembly indicating that L. monocytogenes utilizes a cellular rho dependent mechanism in this process. Previous ArticleNext Article Volume 12Issue 31 March 1993In this issue RelatedDetailsLoading ..." @default.
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• W33138032 date "1993-03-01" @default.
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• W33138032 title "A chimeric toxin to study the role of the 21 kDa GTP binding protein rho in the control of actin microfilament assembly." @default.
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• W33138032 doi "https://doi.org/10.1002/j.1460-2075.1993.tb05733.x" @default.
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