Matches in SemOpenAlex for { <https://semopenalex.org/work/W331401259> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W331401259 abstract "Mutational analyses of cancer genomes have identified novel candidate cancer genes with hitherto unknown function in cancer. To enable phenotyping of mutations in such genes, we have developed a scalable technology for gene knock-in and knock-out in human somatic cells based on recombination-mediated construct generation and a computational tool to design gene targeting constructs. Using this technology, we have generated somatic cell knock-outs of the putative cancer genes ZBED6 and DIP2C in human colorectal cancer cells. In ZBED6-/- cells complete loss of functional ZBED6 was validated and loss of ZBED6 induced the expression of IGF2. Whole transcriptome and ChIP-seq analyses revealed relative enrichment of ZBED6 binding sites at upregulated genes as compared to downregulated genes. The functional annotation of differentially expressed genes revealed enrichment of genes related to cell cycle and cell proliferation and the transcriptional modulator ZBED6 affected the cell growth and cell cycle of human colorectal cancer cells. In DIP2C-/-cells, transcriptome sequencing revealed 780 differentially expressed genes as compared to their parental cells including the tumour suppressor gene CDKN2A. The DIP2C regulated genes belonged to several cancer related processes such as angiogenesis, cell structure and motility. The DIP2C-/-cells were enlarged and grew slower than their parental cells. To be able to directly compare the phenotypes of mutant KRAS and BRAF in colorectal cancers, we have introduced a KRASG13D allele in RKO BRAFV600E/-/-/ cells. The expression of the mutant KRAS allele was confirmed and anchorage independent growth was restored in KRASG13D cells. The differentially expressed genes both in BRAF and KRAS mutant cells included ERBB, TGFB and histone modification pathways. Together, the isogenic model systems presented here can provide insights to known and novel cancer pathways and can be used for drug discovery." @default.
- W331401259 created "2016-06-24" @default.
- W331401259 creator A5012982051 @default.
- W331401259 creator A5015696485 @default.
- W331401259 creator A5042386888 @default.
- W331401259 creator A5067622718 @default.
- W331401259 creator A5075726672 @default.
- W331401259 creator A5090818963 @default.
- W331401259 date "2014-01-01" @default.
- W331401259 modified "2023-09-23" @default.
- W331401259 title "The transcriptional modulator ZBED6 regulates cell cycle and growth of human colorectal cancer cells" @default.
- W331401259 hasPublicationYear "2014" @default.
- W331401259 type Work @default.
- W331401259 sameAs 331401259 @default.
- W331401259 citedByCount "0" @default.
- W331401259 crossrefType "journal-article" @default.
- W331401259 hasAuthorship W331401259A5012982051 @default.
- W331401259 hasAuthorship W331401259A5015696485 @default.
- W331401259 hasAuthorship W331401259A5042386888 @default.
- W331401259 hasAuthorship W331401259A5067622718 @default.
- W331401259 hasAuthorship W331401259A5075726672 @default.
- W331401259 hasAuthorship W331401259A5090818963 @default.
- W331401259 hasConcept C104317684 @default.
- W331401259 hasConcept C121608353 @default.
- W331401259 hasConcept C134305767 @default.
- W331401259 hasConcept C150194340 @default.
- W331401259 hasConcept C162317418 @default.
- W331401259 hasConcept C2777933648 @default.
- W331401259 hasConcept C2778265714 @default.
- W331401259 hasConcept C2781187634 @default.
- W331401259 hasConcept C29537977 @default.
- W331401259 hasConcept C501734568 @default.
- W331401259 hasConcept C502942594 @default.
- W331401259 hasConcept C526805850 @default.
- W331401259 hasConcept C54355233 @default.
- W331401259 hasConcept C555283112 @default.
- W331401259 hasConcept C62112901 @default.
- W331401259 hasConcept C86339819 @default.
- W331401259 hasConcept C86803240 @default.
- W331401259 hasConcept C96232424 @default.
- W331401259 hasConceptScore W331401259C104317684 @default.
- W331401259 hasConceptScore W331401259C121608353 @default.
- W331401259 hasConceptScore W331401259C134305767 @default.
- W331401259 hasConceptScore W331401259C150194340 @default.
- W331401259 hasConceptScore W331401259C162317418 @default.
- W331401259 hasConceptScore W331401259C2777933648 @default.
- W331401259 hasConceptScore W331401259C2778265714 @default.
- W331401259 hasConceptScore W331401259C2781187634 @default.
- W331401259 hasConceptScore W331401259C29537977 @default.
- W331401259 hasConceptScore W331401259C501734568 @default.
- W331401259 hasConceptScore W331401259C502942594 @default.
- W331401259 hasConceptScore W331401259C526805850 @default.
- W331401259 hasConceptScore W331401259C54355233 @default.
- W331401259 hasConceptScore W331401259C555283112 @default.
- W331401259 hasConceptScore W331401259C62112901 @default.
- W331401259 hasConceptScore W331401259C86339819 @default.
- W331401259 hasConceptScore W331401259C86803240 @default.
- W331401259 hasConceptScore W331401259C96232424 @default.
- W331401259 hasLocation W3314012591 @default.
- W331401259 hasOpenAccess W331401259 @default.
- W331401259 hasPrimaryLocation W3314012591 @default.
- W331401259 hasRelatedWork W1533462288 @default.
- W331401259 hasRelatedWork W1973890021 @default.
- W331401259 hasRelatedWork W1989742225 @default.
- W331401259 hasRelatedWork W1995608074 @default.
- W331401259 hasRelatedWork W2084126480 @default.
- W331401259 hasRelatedWork W2086177514 @default.
- W331401259 hasRelatedWork W2087638253 @default.
- W331401259 hasRelatedWork W2088935942 @default.
- W331401259 hasRelatedWork W2114893781 @default.
- W331401259 hasRelatedWork W2120166976 @default.
- W331401259 hasRelatedWork W2127020291 @default.
- W331401259 hasRelatedWork W2152411486 @default.
- W331401259 hasRelatedWork W2346083605 @default.
- W331401259 hasRelatedWork W2886355294 @default.
- W331401259 hasRelatedWork W2944232224 @default.
- W331401259 hasRelatedWork W2948132993 @default.
- W331401259 hasRelatedWork W3187147019 @default.
- W331401259 hasRelatedWork W3190647696 @default.
- W331401259 hasRelatedWork W562615925 @default.
- W331401259 hasRelatedWork W981233335 @default.
- W331401259 isParatext "false" @default.
- W331401259 isRetracted "false" @default.
- W331401259 magId "331401259" @default.
- W331401259 workType "article" @default.