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- W333157095 abstract "This chapter presents an overview of a biosensor to detect organophosphorus insecticides. A new potentiometric assay of organophosphorous compounds (OPC) in water and organic media is developed. The assay is based on application of polyvinyl alcohol) cryogel (PVAG)-entrapped E. coli DH-5α bacteria featuring a high-activity organophosphate hydrolase (OPH). OPH catalyzes the hydrolysis of paraoxon and its analogs to release protons, the concentration of which is proportional to the amount of hydrolyzed substrate. Measurements are performed in 0.5 mM glycine buffer (pH 9.0). In the assay, the cells immobilized in PVAG-beads (diameter 0.7 mm) or films, are placed into a unit equipped with a combined pH-electrode and a mixing device. When organophosphorous compounds are assayed in organic solvents, the assay is conducted in a biphasic system. The assay was elaborated by using such widely known pesticides, as paroxon, paration, and cumaphos. The linear dependence of pH dynamics through hydrolysis of the pesticides by the immobilized cells was observed in the range 10 −7 –10 −5 M. The assay time is 5 min. The immobilized cells are usable in the assay repeatedly. The activity of the immobilized cells on storage in 50 mM phosphatebuffer (pH 7.2) containing CO 2+ remained unchanged for not less than 2 months." @default.
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- W333157095 date "1994-01-01" @default.
- W333157095 modified "2023-09-23" @default.
- W333157095 title "Biosensor to Detect Organophosphorus Insecticides" @default.
- W333157095 doi "https://doi.org/10.1016/b978-1-85617-242-4.50262-7" @default.
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