Matches in SemOpenAlex for { <https://semopenalex.org/work/W33349314> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W33349314 abstract "74 Objectives: Curcumin (CUR) is known to be anitinflammatory, antiamyloidogenic and anticarcinogenic and is known to reduce formation of β-amyloid (Aβ) plaques. CUR binds to Aβ plaques with Ki= 0.2 nM (Ryu et al., 2006). As a PET imaging agent based on CUR, we report: 1. Radiosynthesis of O-18F-fluoropropyl curcumin (18F-FPCur) in a two-step reaction, and 2. Binding of 18F-FPCur in transgenic mouse models Tg2576 (Aβ plaques) and 3xTg triple transgenic (expressing Aβ plaques and neurofibrillary tangles). Methods: 1-Bromo-3-18F-fluoropropane (18F-BFP)was prepared by reacting 1,3-dibromopropane with 18F-fluoride at 90°C in CH3CN for 20 mins. CUR was reacted with 18F-BFP in THF/K2CO3 at 80°C for 30 mins, purified on RP-HPLC (C18, CH3CN, CH3OH, H2O, CH3CO2H 41:23:36:1;1.5 ml/min). Binding to rat brain slices of 18F-FPCur was carried out at 37°C in 0.1 M TBS buffer containing 0.5% Tween, 0.1% BSA, pH 7.4 for 1hr. Sagittal brain sections (10 μm) were obtained from two transgenic models, Tg2576 and 3xTg and wild-type (WT) mice of comparable age. Binding of 18F-FPCur was determined by autroradiograms analyzed by OptiQuant Image Software. Adjacent mice brain sections were immunostained with an anti-Aβ antibody and thioflavin to confirm presence of Aβ plaques. Results: Radiolabeling and isolation of 18F-BFP as a THF solution occurred in >40% radiochemical yield in 40 mins. Radiolabeling of CUR occurred in low yields- approx. 2-3% of the radioactivity computed from collected HPLC fractions. A protected precursor molecule for radiolabeling is underway. Rat brain slices had low levels of nonspecific binding. Transgenic mice brain sections revealed significant amount of binding in areas consistent with the presence of Aβ plaques. Cerebellum (Cb) was used as a reference region- areas rich in Aβ plaques such as hippocampus (Hp), cortex (Ctx) and brain stem (BS) showed greater 18F-FPCur binding: For 3xTg: Hp/Cb= 2.6; Ctx/Cb= 1.23; BS/Cb= 2.7; and for Tg2576: Hp/Cb=1.9; Ctx/Cb= 1.8; BS/Cb=1.2. Thus, cortical regions of Tg2576 model contain higher degree of Aβ plaques. Conclusions: 18F-FPCur was prepared in a 2-step reaction. Methods to improve yields are currently underway. Binding of 18F-FPCur in transgenic mouse models indicates potential as a PET imaging agent." @default.
- W33349314 created "2016-06-24" @default.
- W33349314 creator A5058964174 @default.
- W33349314 creator A5070326390 @default.
- W33349314 creator A5070892687 @default.
- W33349314 creator A5073234292 @default.
- W33349314 creator A5079119372 @default.
- W33349314 creator A5079184738 @default.
- W33349314 date "2007-05-01" @default.
- W33349314 modified "2023-09-26" @default.
- W33349314 title "18F-Fluoropropyl curcumin: A potential PET tracer for imaging inflammation and Aβ-plaques" @default.
- W33349314 hasPublicationYear "2007" @default.
- W33349314 type Work @default.
- W33349314 sameAs 33349314 @default.
- W33349314 citedByCount "0" @default.
- W33349314 crossrefType "journal-article" @default.
- W33349314 hasAuthorship W33349314A5058964174 @default.
- W33349314 hasAuthorship W33349314A5070326390 @default.
- W33349314 hasAuthorship W33349314A5070892687 @default.
- W33349314 hasAuthorship W33349314A5073234292 @default.
- W33349314 hasAuthorship W33349314A5079119372 @default.
- W33349314 hasAuthorship W33349314A5079184738 @default.
- W33349314 hasConcept C102230213 @default.
- W33349314 hasConcept C104317684 @default.
- W33349314 hasConcept C13965031 @default.
- W33349314 hasConcept C141035611 @default.
- W33349314 hasConcept C150903083 @default.
- W33349314 hasConcept C153911025 @default.
- W33349314 hasConcept C185592680 @default.
- W33349314 hasConcept C207001950 @default.
- W33349314 hasConcept C2775842073 @default.
- W33349314 hasConcept C2776970183 @default.
- W33349314 hasConcept C2778172261 @default.
- W33349314 hasConcept C2778250585 @default.
- W33349314 hasConcept C2989005 @default.
- W33349314 hasConcept C55493867 @default.
- W33349314 hasConcept C71924100 @default.
- W33349314 hasConcept C86803240 @default.
- W33349314 hasConceptScore W33349314C102230213 @default.
- W33349314 hasConceptScore W33349314C104317684 @default.
- W33349314 hasConceptScore W33349314C13965031 @default.
- W33349314 hasConceptScore W33349314C141035611 @default.
- W33349314 hasConceptScore W33349314C150903083 @default.
- W33349314 hasConceptScore W33349314C153911025 @default.
- W33349314 hasConceptScore W33349314C185592680 @default.
- W33349314 hasConceptScore W33349314C207001950 @default.
- W33349314 hasConceptScore W33349314C2775842073 @default.
- W33349314 hasConceptScore W33349314C2776970183 @default.
- W33349314 hasConceptScore W33349314C2778172261 @default.
- W33349314 hasConceptScore W33349314C2778250585 @default.
- W33349314 hasConceptScore W33349314C2989005 @default.
- W33349314 hasConceptScore W33349314C55493867 @default.
- W33349314 hasConceptScore W33349314C71924100 @default.
- W33349314 hasConceptScore W33349314C86803240 @default.
- W33349314 hasLocation W333493141 @default.
- W33349314 hasOpenAccess W33349314 @default.
- W33349314 hasPrimaryLocation W333493141 @default.
- W33349314 hasRelatedWork W178002768 @default.
- W33349314 hasRelatedWork W1992243124 @default.
- W33349314 hasRelatedWork W2005329402 @default.
- W33349314 hasRelatedWork W2013374328 @default.
- W33349314 hasRelatedWork W2018512343 @default.
- W33349314 hasRelatedWork W2043494023 @default.
- W33349314 hasRelatedWork W2082411172 @default.
- W33349314 hasRelatedWork W2100132823 @default.
- W33349314 hasRelatedWork W2214901722 @default.
- W33349314 hasRelatedWork W2280796408 @default.
- W33349314 hasRelatedWork W2535666629 @default.
- W33349314 hasRelatedWork W2554972700 @default.
- W33349314 hasRelatedWork W2599268966 @default.
- W33349314 hasRelatedWork W2605025925 @default.
- W33349314 hasRelatedWork W2624828552 @default.
- W33349314 hasRelatedWork W2799937258 @default.
- W33349314 hasRelatedWork W2895328053 @default.
- W33349314 hasRelatedWork W3091699344 @default.
- W33349314 hasRelatedWork W3171998967 @default.
- W33349314 hasRelatedWork W3175460446 @default.
- W33349314 hasVolume "48" @default.
- W33349314 isParatext "false" @default.
- W33349314 isRetracted "false" @default.
- W33349314 magId "33349314" @default.
- W33349314 workType "article" @default.