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- W334753205 abstract "This chapter discusses the depletion of mononuclear phagocytes, focusing on the pitfalls in the use of carbonyl iron, carrageenan, silica, trypan blue, or antimononuclear phagocyte serum. The removal of mononuclear phagocytes with carbonyl iron theoretically depends on the phagocytic uptake of iron, after which iron-containing cells are removed in a magnetic field. Carbonyl iron has been found to remove up to 98% of the macrophages from thioglycollate-elicited peritoneal cells. However, the overall recovery of cells in these studies has been low. Such low recoveries are a major problem with the technique as iron particles often are entrapped in the clumps of cells or adhere to the surfaces of nonphagocytic cells. Carrageenan is dissolved in phosphate-buffered saline by heating to boiling. The final preparation may be sterilized by autoclaving. The usual routes of administration are intravenous or intraperitoneal. The intravenous dose is 0.5 ml of a 1 mg/ml solution of carrageenan. Trypan blue has been reported to interfere with the rejection of allogeneic skin and tumors as well as the elimination of antigenic, syngeneic neoplasms." @default.
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- W334753205 date "1981-01-01" @default.
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- W334753205 title "DEPLETION OF MONONUCLEAR PHAGOCYTES: PITFALLS IN THE USE OF CARBONYL IRON, CARRAGEENAN, SILICA, TRYPAN BLUE, OR ANTIMONONUCLEAR PHAGOCYTE SERUM" @default.
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- W334753205 doi "https://doi.org/10.1016/b978-0-12-044220-1.50032-5" @default.
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