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- W335422306 abstract "Abstract Strains of E . coli with fusions between the lac structural genes and the promoter region of the fumarate reductase system were constructed from a parental strain deleted in the native lac operon. Like fumarate reductase in wild-type cells, β-galactosidase in these fusion strains is inducible by fumarate, but only under anaerobic conditions. From one of these strains, three classes of mutants altered in the expression of the hybrid operon were isolated. By anaerobic selection for growth on lactose in the absence of fumarate, mutants that synthesize β-galactosidase constitutively both aerobically and anaerobically were obtained. By aerobic selection for growth on lactose in the presence of fumarate, mutants that are inducible in the enzyme both aerobically and anaerobically and mutants that are inducible in the enzyme only aerobically were obtained. The regulatory behaviors of the mutants studied suggest that substrate and respiratory control of the expression of the fumarate reductase complex are mechanistically connected." @default.
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- W335422306 date "1979-12-01" @default.
- W335422306 modified "2023-10-16" @default.
- W335422306 title "Use of lac operon fusions to isolate Escherichiacoli mutants with altered expression of the fumarate reductase system in response to substrate and respiratory controls" @default.
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- W335422306 doi "https://doi.org/10.1016/0006-291x(79)91217-8" @default.
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