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- W336502018 abstract "According to the published sequence of African swine fever virus(ASFV) P54 gene in GenBankTM,two pairs of primers were designed and synthesized.The P54 gene and P54q gene without transmembrane domain was amplified by PCR from genomic DNA of ASFV,and cloned them into pET28b to construct recombinant plasmids pET-P54 and pET-P54q,respectively.After sequencing,the two plasmids were transformed into Escherichia coli BL21(DE3) and induced with isopropyl-β-D-thiogalactoside(IPTG) for expressing.SDS-PAGE analysis showed that the transmembrane domain deletion P54q with 24.5 ku in molecular mass was successfully expressed in a soluble protein form.The recombinant protein P54q purified with HisLink Protein Purification Resin could reacted with positive sera against ASFV by Western-blot,indicating that P54q protein had reactogenicity." @default.
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- W336502018 date "2009-01-01" @default.
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- W336502018 title "Prokaryotic expression of African swine fever virus P54 gene." @default.
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