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- W336725901 abstract "The Antarctic ocean is a stable environment, especially concerning its constantly lowtemperatures that are not subjected to great seasonal changes. The resident animals are welladapted to a narrow temperature range and usually characterised as stenothermal, thus beingvulnerable to temperature changes.This also accounts for the antarctic eelpout Pachycara brachycephalum, that has been studiedin the course of this Bachelor-thesis for temperature-dependent long-term changes in acidbase-regulation and metabolism. Experiments were conducted on samples of white muscletissue of animals that were previously incubated to six dfferent temperatures, ranging from -1to 9°C, for two months. This range covers the known boundaries of temperature-tolerance ofthe species investigated. Changes in intracellular pH (pHi), c(CO2), [HCO3-] and P(CO2) inwhite muscle tissue were determined using the homogenate technique. Through the analysisof 1H-NMR-spectra, I was able to look for changes in the relative concentrations of lactate,succinate and imidazole, in order to gain information on anaerobic metabolism and bufferingcharacteristics of the cytoplasm.A decline in pHi of -0,018°C-1was detected between 0 and 3°C, that coincides with short-termstudies on the same tissue and indicates alphastat regulation. pHi rose predominantly between3 and 9°C; and an increased CO2-content was found between 7 and 9°C. These findings differfrom previously investigated short-term patterns and indicate that alphastat regulation mightnot be sustainable on longer timescales.No change was found in the relative concentrations of anaerobic metabolites lactate andsuccinate, whilst the imidazole-concentration dropped already at 3°C and remained constantlylow despite rising temperatures. The differences in pHi-regulation between long-term andacute adaptations could be related to the lack of this important intracellular buffer and to a risein tissue bicarbonate concentration. The aerobic capacity of the animal is linked to its thermal tolerance. Earlier studies showed that oxygen supply is reduced at about 6°C resulting in negative growth rates at thistemperature and above. Optimal growth has already been measured as well, ranging between3 to 4°C and correlating with the lowest levels of gene-expression in hepatocytes. Up to theselimits, pHi changed according to the proposed alphastat-Hypothesis. With increasingtemperatures, first pHi, then CO2-content deviate from the alphastat-pattern and thiscorresponds to the before mentioned reduction in aerobic capacity. Therefore the capability touse aerobic metabolism seems to be defining the efficiency of acid-base regulation." @default.
- W336725901 created "2016-06-24" @default.
- W336725901 creator A5039688164 @default.
- W336725901 date "2013-08-01" @default.
- W336725901 modified "2023-09-27" @default.
- W336725901 title "Temperature dependent pH and metabolic regulation in Antarctic fish" @default.
- W336725901 hasPublicationYear "2013" @default.
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