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- W337585719 abstract "A radioimmunoassay for the determination of urinary aldosterone-18- glucuronide was developed for the clinical laboratory. Ten ml of urine is pre- extracted with methylene chloride and then submitted to acid hydrolysis. This is followed by a second methylene chloride extraction. The aldosterone concentration in these extracts is determined with antisera obtained from rabbits immunized with an aldosterone-oxime bovine gamma globulin conjugate, In view of the high recovery of STA1,2/sup -3/H! aldosterone, no internal indicator standands were used. The sensitivity of the overall assay was 0.44 mu g/24 h. The coefficient of variation was 3.7% within a single assay and 13.8% for multiple assays. The specificity of the method was examined by comparison with a double isotope dilution derivative method and by simultaneous use of six different antisera. Under normal sodium intake the urinary excretion of the acid- labile aldosterone-18-glucuronide ranged from 4.0 to 13.0 mu g 24 h in nine normal male subjects. (auth)" @default.
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- W337585719 date "1973-01-01" @default.
- W337585719 modified "2023-09-24" @default.
- W337585719 title "Radioimmunoassay for aldosterone without chromatography. Determination of urinary 18-glucuronide excretion" @default.
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