Matches in SemOpenAlex for { <https://semopenalex.org/work/W337601332> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W337601332 abstract "Background: The cellular effects of anesthetics is poorly known. The GABAA receptor has been suggested as the main target for most anesthetics. In previous studies we have shown that propofol tyresine phosphorylates the GABAA receptor s subunit, increases intracellular calcium and changes the actin morphology of neurons.Aim: To investigate the effects of the volatile anesthetics sevoflurane, isoflurane and nitmus oxide on changes in [Ca2+]i tyrosine phosphorylation and actin morphology in cultured rat neurons.Methods: Western blotting (WB) was used to visualize tyrosine phosphorylated proteins. Fluorescence microscopy after rhodamine-phalloidin labelling of actin was used to calculate the number of actin ring structures eaused by sevoflurane. Intracellular calcium was measured with the calcium-binding probe Fura-2 on single cells.Results: A protein of approx. 60 kDa increased dose-dependently in tyresine phosphorylation by sevoflurane in the membrane and cytoskeletal fractions, and was simultaneausly reduced in the cytosol. Isoflurane instead increased the tyresine phosphorylation of the same protein in the cytosol with only a slight increase in the membrane and no changes in the cytoskeletal fraction. Nitrous oxide did not cause any changes campared to air in the cytosol and was not detectable in the membrane. However, in the cytoskeletal fraction, the increase in tyrosine phosphorylation was high compared to air. Sevoflurane but not nitrous oxide or air increased the [Ca2+]i· Sevoflurane also eaused actin ring structures with a maximum after 20 minutes.Conclusion: Sevoflurane, isoflurane and nitrous oxide all have different signal pathways. The 60 kDa protein is probably the GABAA receptor s subunit. According to the changes in tyrosine phosphorylation, changes in actin morphology and intracellular calcium, sevoflurane behaves most like the intravenous anesthetic propofol." @default.
- W337601332 created "2016-06-24" @default.
- W337601332 creator A5032589605 @default.
- W337601332 date "2003-01-01" @default.
- W337601332 modified "2023-09-27" @default.
- W337601332 title "Cellular mechanisms of anaesthetic agents" @default.
- W337601332 hasPublicationYear "2003" @default.
- W337601332 type Work @default.
- W337601332 sameAs 337601332 @default.
- W337601332 citedByCount "0" @default.
- W337601332 crossrefType "journal-article" @default.
- W337601332 hasAuthorship W337601332A5032589605 @default.
- W337601332 hasConcept C11960822 @default.
- W337601332 hasConcept C12554922 @default.
- W337601332 hasConcept C125705527 @default.
- W337601332 hasConcept C142669718 @default.
- W337601332 hasConcept C1491633281 @default.
- W337601332 hasConcept C178790620 @default.
- W337601332 hasConcept C181199279 @default.
- W337601332 hasConcept C185592680 @default.
- W337601332 hasConcept C2776359302 @default.
- W337601332 hasConcept C2777553839 @default.
- W337601332 hasConcept C55493867 @default.
- W337601332 hasConcept C86803240 @default.
- W337601332 hasConcept C95444343 @default.
- W337601332 hasConcept C98539663 @default.
- W337601332 hasConceptScore W337601332C11960822 @default.
- W337601332 hasConceptScore W337601332C12554922 @default.
- W337601332 hasConceptScore W337601332C125705527 @default.
- W337601332 hasConceptScore W337601332C142669718 @default.
- W337601332 hasConceptScore W337601332C1491633281 @default.
- W337601332 hasConceptScore W337601332C178790620 @default.
- W337601332 hasConceptScore W337601332C181199279 @default.
- W337601332 hasConceptScore W337601332C185592680 @default.
- W337601332 hasConceptScore W337601332C2776359302 @default.
- W337601332 hasConceptScore W337601332C2777553839 @default.
- W337601332 hasConceptScore W337601332C55493867 @default.
- W337601332 hasConceptScore W337601332C86803240 @default.
- W337601332 hasConceptScore W337601332C95444343 @default.
- W337601332 hasConceptScore W337601332C98539663 @default.
- W337601332 hasLocation W3376013321 @default.
- W337601332 hasOpenAccess W337601332 @default.
- W337601332 hasPrimaryLocation W3376013321 @default.
- W337601332 hasRelatedWork W1508352585 @default.
- W337601332 hasRelatedWork W1980846708 @default.
- W337601332 hasRelatedWork W1990508774 @default.
- W337601332 hasRelatedWork W2001485902 @default.
- W337601332 hasRelatedWork W2010573112 @default.
- W337601332 hasRelatedWork W2017889315 @default.
- W337601332 hasRelatedWork W2022276053 @default.
- W337601332 hasRelatedWork W2063795429 @default.
- W337601332 hasRelatedWork W2069491024 @default.
- W337601332 hasRelatedWork W2091320669 @default.
- W337601332 hasRelatedWork W2101931421 @default.
- W337601332 hasRelatedWork W2104241104 @default.
- W337601332 hasRelatedWork W2109481204 @default.
- W337601332 hasRelatedWork W2117155496 @default.
- W337601332 hasRelatedWork W2146380955 @default.
- W337601332 hasRelatedWork W2400888006 @default.
- W337601332 hasRelatedWork W2466997536 @default.
- W337601332 hasRelatedWork W2473448248 @default.
- W337601332 hasRelatedWork W2592568272 @default.
- W337601332 hasRelatedWork W2887001450 @default.
- W337601332 isParatext "false" @default.
- W337601332 isRetracted "false" @default.
- W337601332 magId "337601332" @default.
- W337601332 workType "article" @default.