FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3376234> ?p ?o ?g. }

• W3376234 abstract "Frizzled genes encode a family of Wnt ligand receptors, which have a conserved cysteine-rich Wnt binding domain and include both transmembrane and secreted forms. Work by others has shown that experimental perturbation of Wnt signaling results in aberrant hair formation, hair growth and hair structure. However, to date there is no information on the contribution of individual Frizzled proteins to hair development. We now report that Frizzled-3 (Fzd3) expression in skin is restricted to the epidermis and to the developing hair follicle. Northern analysis on total mouse skin mRNA revealed a single Fzd3 transcript of 3.7 kb. RT-PCR and in situ hybridization analysis revealed Fzd3 expression in epidermal and hair follicle keratinocytes. Fzd3 transcripts are first detected in discrete foci in the developing epidermis of 13 day embryos and later in the hair follicle placodes of 15 day embryos, suggesting a role for this Frizzled isoform in follicle development. In 17 day embryos and 1 day old newborn mice Fzd3 expression is limited to suprabasal keratinocytes and is not seen in pelage follicles until 3 days post partum. In 7 day old neonatal skin, Fzd3 is expressed throughout the epidermis and in the outer cell layers of hair follicles. We have also identified the mRNA encoding human Frizzled-3 (FZD3) in epidermal keratinocytes and in the HaCaT keratinocyte cell line. FZD3 mRNA encodes a 666 amino-acid protein with 97.8% identity to the mouse protein. The FZD3 gene was mapped using a radiation-hybrid cell line panel to the short arm of chromosome 8 (8p21) between the markers WI-1172 and WI-8496 near the loci for the Hypotrichosis of Marie Unna and Hairless genes. The exon-intron organization and sequences of the exon-intron boundaries of the mouse Frizzled-3 (mFzd3) transmembrane receptor gene have been determined. The mFzd3 gene comprises 7 exons and shows organizational conservation with the human homolog (hFZD3) within the coding region but differs in the numbers of 5' non-coding exon. Interestingly, the 5'-untranslated region (5'-UTR) of both mouse and human transcripts contain several upstream ORFs that are conserved in sequence and position. This study has also identified several alternative spliced transcripts, which are predicted to encode soluble variants that lack the transmembrane and C-terminal domain of Frizzled-3 protein (Fzd3). The mFzd3a isoform which is structurally similar to sFRP/Frzb proteins may function to prevent formation of the Frizzled-Wnt complex and inhibit signaling. The mFzd3d and mFzd3e isoforms which are structurally similar to FZD4S (Sagara et al., 2001) may heretodimerize with mFzd3b, suggesting that Frizzled activity requires receptor oligomerization." @default.
• W3376234 created "2016-06-24" @default.
• W3376234 creator A5046596292 @default.
• W3376234 date "2003-01-01" @default.
• W3376234 modified "2023-09-26" @default.
• W3376234 title "Characterization of mouse frizzled-3 gene and identification of human homolog in keratinocytes" @default.
• W3376234 hasPublicationYear "2003" @default.
• W3376234 type Work @default.
• W3376234 sameAs 3376234 @default.
• W3376234 citedByCount "0" @default.
• W3376234 crossrefType "journal-article" @default.
• W3376234 hasAuthorship W3376234A5046596292 @default.
• W3376234 hasConcept C104317684 @default.
• W3376234 hasConcept C105702510 @default.
• W3376234 hasConcept C107300179 @default.
• W3376234 hasConcept C118995209 @default.
• W3376234 hasConcept C137620995 @default.
• W3376234 hasConcept C150194340 @default.
• W3376234 hasConcept C2776458125 @default.
• W3376234 hasConcept C2777074287 @default.
• W3376234 hasConcept C2777216303 @default.
• W3376234 hasConcept C54355233 @default.
• W3376234 hasConcept C62478195 @default.
• W3376234 hasConcept C81439078 @default.
• W3376234 hasConcept C81885089 @default.
• W3376234 hasConcept C86803240 @default.
• W3376234 hasConcept C95444343 @default.
• W3376234 hasConceptScore W3376234C104317684 @default.
• W3376234 hasConceptScore W3376234C105702510 @default.
• W3376234 hasConceptScore W3376234C107300179 @default.
• W3376234 hasConceptScore W3376234C118995209 @default.
• W3376234 hasConceptScore W3376234C137620995 @default.
• W3376234 hasConceptScore W3376234C150194340 @default.
• W3376234 hasConceptScore W3376234C2776458125 @default.
• W3376234 hasConceptScore W3376234C2777074287 @default.
• W3376234 hasConceptScore W3376234C2777216303 @default.
• W3376234 hasConceptScore W3376234C54355233 @default.
• W3376234 hasConceptScore W3376234C62478195 @default.
• W3376234 hasConceptScore W3376234C81439078 @default.
• W3376234 hasConceptScore W3376234C81885089 @default.
• W3376234 hasConceptScore W3376234C86803240 @default.
• W3376234 hasConceptScore W3376234C95444343 @default.
• W3376234 hasLocation W33762341 @default.
• W3376234 hasOpenAccess W3376234 @default.
• W3376234 hasPrimaryLocation W33762341 @default.
• W3376234 hasRelatedWork W1542375841 @default.
• W3376234 hasRelatedWork W1991221065 @default.
• W3376234 hasRelatedWork W1997309506 @default.
• W3376234 hasRelatedWork W1998326294 @default.
• W3376234 hasRelatedWork W2012335330 @default.
• W3376234 hasRelatedWork W2014052142 @default.
• W3376234 hasRelatedWork W2014627257 @default.
• W3376234 hasRelatedWork W2019540978 @default.
• W3376234 hasRelatedWork W2024935572 @default.
• W3376234 hasRelatedWork W2040446613 @default.
• W3376234 hasRelatedWork W2040888445 @default.
• W3376234 hasRelatedWork W2051601693 @default.
• W3376234 hasRelatedWork W2063229072 @default.
• W3376234 hasRelatedWork W2065852799 @default.
• W3376234 hasRelatedWork W2071129803 @default.
• W3376234 hasRelatedWork W2074177228 @default.
• W3376234 hasRelatedWork W2084275797 @default.
• W3376234 hasRelatedWork W2092880556 @default.
• W3376234 hasRelatedWork W2168002381 @default.
• W3376234 hasRelatedWork W2390218749 @default.
• W3376234 isParatext "false" @default.
• W3376234 isRetracted "false" @default.
• W3376234 magId "3376234" @default.
• W3376234 workType "article" @default.