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• W341586691 abstract "Aims: The escalating demands for traditional fossil fuels with unsecured deliverance and issues of climate change compel the researchers to develop alternative fuels like bioethanol. This study examines the prospect of biofuel production from high carbohydrate containing lignocellulosic material, e.g. sugarcane bagasse through biological means. Methodology and Results: Cellulolytic enzymes were collected from the culture filtrate of thermotolerant Trichoderma viride grown on variously pretreated sugarcane bagasse. CMCase and FPase enzyme activities were determined as a measure of suitable substrate pretreatment and opt imum condition for cellulolytic enzyme production. The highest CMCase and FPase activity was found to be 1.217 U/ml and 0.109 U/ml respectively under the production conditions of 200 rpm, pH 4.0 and 50 °C using steamed NaOH treated bagasse as substrate. SEM was carried out to compare and confirm the activity of cellulolytic enzymes on sugarcane bagasse. Saccharification of pretreated bag asse was carried out with crude enzymes together using a twofactor experimental design. Under optimized conditions the pretreated bagasse was saccharified up to 42.7 % in 24 h. The hydrolysate was concentrated by heating to suitable concentration and then used for fermentation by an indigenous isolate of Saccharomyces cerevisiae . With 50 and 80 % brix containing liquor the concentration of alcohol was 0.579 % and 1.15 % respectively. Conclusion, significance and impact of study: This is the first report in Bangladesh for the production of cellulosic ethanol using local isolates. Though the rate of alcohol production was very low, a great impetus in this field can maximize the production thereby meet the demand for fuel in future." @default.
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• W341586691 date "2012-06-01" @default.
• W341586691 modified "2023-09-25" @default.
• W341586691 title "Saccharification of Sugarcane Bagasse by Enzymatic Treatment for bioethanol production" @default.
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• W341586691 doi "https://doi.org/10.21161/mjm.03412" @default.
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