Matches in SemOpenAlex for { <https://semopenalex.org/work/W34280241> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W34280241 abstract "To facilitate the practical application of highly efficient semi-automated methods for general application in genomic analyses, we have developed a fluorescence-based marker resource. Ninety highly polymorphic simple tandem repeat markers were combined to provide a rapid, accurate, and highly efficient initial genome-wide screening system. These markers are spaced on average every 33 recombination units, with a mean heterozygosity of 81% (range 65-94%), covering 22 autosomes and the X and Y chromosomes. Less than 3% of the genome lies beyond 30 cM of the nearest marker. Markers were placed in a vertical ladder that we have termed a SET according to the size of the PCR fragments they produce during electrophoresis. Each SET was designed to avoid overlap between loci during gel separations to assure accuracy when scoring genotypes. We have constructed 15 SETS of markers. Three SETS, each labelled with one of three fluors, were combined into what we have termed a GROUP, which is co-electrophoresed with internal size standards that are labelled with a fourth flour. Five GROUPS of markers were assembled that contain a total of 15 SETS of markers. Each GROUP cover 18 regions of the genome that can be detected simultaneously, since this genomic analysis systemmore » is fully compatible with automated fragment analyzers using simultaneous four-color fluorescence-based detection systems. This allows for multiplex detection and a throughput of 1,944 genotypes daily per instrument. This system will be highly beneficial in a number of clinical and research applications including: linkage, cancer genetics, forensics, and cytogenetics.« less" @default.
- W34280241 created "2016-06-24" @default.
- W34280241 creator A5006490775 @default.
- W34280241 creator A5061316083 @default.
- W34280241 creator A5083507537 @default.
- W34280241 date "1994-09-01" @default.
- W34280241 modified "2023-10-13" @default.
- W34280241 title "Fluorescence-based resource for semi-automated genomic analyses" @default.
- W34280241 hasPublicationYear "1994" @default.
- W34280241 type Work @default.
- W34280241 sameAs 34280241 @default.
- W34280241 citedByCount "0" @default.
- W34280241 crossrefType "journal-article" @default.
- W34280241 hasAuthorship W34280241A5006490775 @default.
- W34280241 hasAuthorship W34280241A5061316083 @default.
- W34280241 hasAuthorship W34280241A5083507537 @default.
- W34280241 hasConcept C104317684 @default.
- W34280241 hasConcept C134925867 @default.
- W34280241 hasConcept C135763542 @default.
- W34280241 hasConcept C141231307 @default.
- W34280241 hasConcept C143589142 @default.
- W34280241 hasConcept C180754005 @default.
- W34280241 hasConcept C190789776 @default.
- W34280241 hasConcept C2781188995 @default.
- W34280241 hasConcept C30481170 @default.
- W34280241 hasConcept C54355233 @default.
- W34280241 hasConcept C61320498 @default.
- W34280241 hasConcept C70721500 @default.
- W34280241 hasConcept C86803240 @default.
- W34280241 hasConceptScore W34280241C104317684 @default.
- W34280241 hasConceptScore W34280241C134925867 @default.
- W34280241 hasConceptScore W34280241C135763542 @default.
- W34280241 hasConceptScore W34280241C141231307 @default.
- W34280241 hasConceptScore W34280241C143589142 @default.
- W34280241 hasConceptScore W34280241C180754005 @default.
- W34280241 hasConceptScore W34280241C190789776 @default.
- W34280241 hasConceptScore W34280241C2781188995 @default.
- W34280241 hasConceptScore W34280241C30481170 @default.
- W34280241 hasConceptScore W34280241C54355233 @default.
- W34280241 hasConceptScore W34280241C61320498 @default.
- W34280241 hasConceptScore W34280241C70721500 @default.
- W34280241 hasConceptScore W34280241C86803240 @default.
- W34280241 hasLocation W342802411 @default.
- W34280241 hasOpenAccess W34280241 @default.
- W34280241 hasPrimaryLocation W342802411 @default.
- W34280241 hasRelatedWork W1208554 @default.
- W34280241 hasRelatedWork W1978043366 @default.
- W34280241 hasRelatedWork W1978417981 @default.
- W34280241 hasRelatedWork W1985778510 @default.
- W34280241 hasRelatedWork W1991243408 @default.
- W34280241 hasRelatedWork W2034838304 @default.
- W34280241 hasRelatedWork W2110091431 @default.
- W34280241 hasRelatedWork W2120512069 @default.
- W34280241 hasRelatedWork W2163010255 @default.
- W34280241 hasRelatedWork W2166694065 @default.
- W34280241 hasRelatedWork W217877204 @default.
- W34280241 hasRelatedWork W2418492248 @default.
- W34280241 hasRelatedWork W2435936460 @default.
- W34280241 hasRelatedWork W246050179 @default.
- W34280241 hasRelatedWork W2596068642 @default.
- W34280241 hasRelatedWork W74069843 @default.
- W34280241 hasRelatedWork W2726447540 @default.
- W34280241 hasRelatedWork W2926353951 @default.
- W34280241 hasRelatedWork W2932905693 @default.
- W34280241 hasRelatedWork W8152447 @default.
- W34280241 hasVolume "55" @default.
- W34280241 isParatext "false" @default.
- W34280241 isRetracted "false" @default.
- W34280241 magId "34280241" @default.
- W34280241 workType "article" @default.