Matches in SemOpenAlex for { <https://semopenalex.org/work/W343191907> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W343191907 abstract "We previously demonstrated enhanced 4-hydroxynonenal (4-HNE) post-translational modification (PTM) of TRPV1 decreases TRPV1 functional expression and contributes to microvascular dysfunction in diabetes. Accordingly, we hypothesized that manipulation of residues associated with 4-HNE PTM would preserve TRPV1 function and restore vascular integrity. 4-HNE decreased capsaicin mediated increases in myocardial blood flow and capsaicin-mediated relaxation in isolated coronary microvessels. TRPV1 functional analysis using electrophysiology revealed blunted capsaicin-mediated currents in the presence of 4-HNE which were reversed by the reactive carbonyl species scavenger aminoguanidine (AGD). Using computational biology, we identified three probable residues, previously identified to be important for oxidative modification, as potential sites for 4-HNE modification. The corresponding residues, C616, C621 and C634, were mutated to alanine (individually and in combination) and subsequently we examined the effects of 4-HNE on TRPV1 currents induced by capsaicin via electrophysiology. The mutation of the three pore cysteine (individually or in combination) abrogated the effects of 4-HNE on capsaicin-mediated currents. These data suggest that TRPV1 is targeted by redox-active substances that directly modulate channel activity at numerous sites in diabetes to decrease TRPV1 functional expression and contribute to microvascular dysfunction. The results obtained demonstrate an optimal redox state is critical for a properly functioning TRPV1 channel." @default.
- W343191907 created "2016-06-24" @default.
- W343191907 creator A5007448245 @default.
- W343191907 creator A5035959153 @default.
- W343191907 creator A5042007281 @default.
- W343191907 creator A5059468684 @default.
- W343191907 date "2015-04-01" @default.
- W343191907 modified "2023-09-26" @default.
- W343191907 title "TRPV1 residues vital to protection against 4‐HNE modification, preservation of channel activity and microvascular function" @default.
- W343191907 doi "https://doi.org/10.1096/fasebj.29.1_supplement.795.8" @default.
- W343191907 hasPublicationYear "2015" @default.
- W343191907 type Work @default.
- W343191907 sameAs 343191907 @default.
- W343191907 citedByCount "0" @default.
- W343191907 crossrefType "journal-article" @default.
- W343191907 hasAuthorship W343191907A5007448245 @default.
- W343191907 hasAuthorship W343191907A5035959153 @default.
- W343191907 hasAuthorship W343191907A5042007281 @default.
- W343191907 hasAuthorship W343191907A5059468684 @default.
- W343191907 hasConcept C12554922 @default.
- W343191907 hasConcept C126322002 @default.
- W343191907 hasConcept C155164980 @default.
- W343191907 hasConcept C160145004 @default.
- W343191907 hasConcept C170493617 @default.
- W343191907 hasConcept C181199279 @default.
- W343191907 hasConcept C185263204 @default.
- W343191907 hasConcept C185592680 @default.
- W343191907 hasConcept C2779201268 @default.
- W343191907 hasConcept C2780051329 @default.
- W343191907 hasConcept C48349386 @default.
- W343191907 hasConcept C55493867 @default.
- W343191907 hasConcept C71924100 @default.
- W343191907 hasConcept C86803240 @default.
- W343191907 hasConcept C95444343 @default.
- W343191907 hasConceptScore W343191907C12554922 @default.
- W343191907 hasConceptScore W343191907C126322002 @default.
- W343191907 hasConceptScore W343191907C155164980 @default.
- W343191907 hasConceptScore W343191907C160145004 @default.
- W343191907 hasConceptScore W343191907C170493617 @default.
- W343191907 hasConceptScore W343191907C181199279 @default.
- W343191907 hasConceptScore W343191907C185263204 @default.
- W343191907 hasConceptScore W343191907C185592680 @default.
- W343191907 hasConceptScore W343191907C2779201268 @default.
- W343191907 hasConceptScore W343191907C2780051329 @default.
- W343191907 hasConceptScore W343191907C48349386 @default.
- W343191907 hasConceptScore W343191907C55493867 @default.
- W343191907 hasConceptScore W343191907C71924100 @default.
- W343191907 hasConceptScore W343191907C86803240 @default.
- W343191907 hasConceptScore W343191907C95444343 @default.
- W343191907 hasIssue "S1" @default.
- W343191907 hasLocation W3431919071 @default.
- W343191907 hasOpenAccess W343191907 @default.
- W343191907 hasPrimaryLocation W3431919071 @default.
- W343191907 hasRelatedWork W1525819881 @default.
- W343191907 hasRelatedWork W1988174555 @default.
- W343191907 hasRelatedWork W2016547570 @default.
- W343191907 hasRelatedWork W2037651260 @default.
- W343191907 hasRelatedWork W2148393333 @default.
- W343191907 hasRelatedWork W2161900438 @default.
- W343191907 hasRelatedWork W2501325194 @default.
- W343191907 hasRelatedWork W2610358825 @default.
- W343191907 hasRelatedWork W3023357719 @default.
- W343191907 hasRelatedWork W4247223114 @default.
- W343191907 hasVolume "29" @default.
- W343191907 isParatext "false" @default.
- W343191907 isRetracted "false" @default.
- W343191907 magId "343191907" @default.
- W343191907 workType "article" @default.