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• W34331475 abstract "For largely unknown reasons severe or moderate diabetes of pregnant rats results in pronounced fetal growth retardation. Therefore, some skeletal growth parameters of fetal rats from streptozotocin diabetic mothers were studied in vivo and in vitro. Two days post conception rats were intravenously injected with 65 mg/kg body weight streptozotocin. On day 20 post conception 8 normal and 8 diabetic rat mothers received 5 μCi 3-H thymidine intraperitoneally. One day later the experiments were terminated. Fetal body weight and body length were significantly (p<0.05–0.001) reduced in the hyperglycaemic rats compared to normal rats, as was the thymidine incorporation into rib cartilage (p<0.02). In the cell culture colony formation from isolated chondrocytes of normal and hyperglycaemic fetuses was determined. Proinsulin, insulin (62.5–250 ng/ml), insulin-like growth factor I and II (6.25–25 ng/ml) significantly (p<0.05–0.001) augmented colony formation in a dose-dependent manner, with the somatomedins being 8 times more effective than proinsulin or insulin. Isolated chondrocytes from hyperglycaemic compared to normal fetuses formed significantly (p<0.05–0.001) fewer colonies in the basal state and in response to all 4 hormones. The results confirm the growth retardation of fetuses from diabetic rat mothers. A reduced responsiveness of chondrocytes from hyperglycaemic fetuses to various growth factors could be demonstrated as compared to cells from normal fetuses." @default.
• W34331475 created "2016-06-24" @default.
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• W34331475 date "1987-02-01" @default.
• W34331475 modified "2023-10-16" @default.
• W34331475 title "Skeletal growth of fetuses from streptozotocin diabetic rat mothers: in vivo and in vitro studies" @default.
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• W34331475 doi "https://doi.org/10.1007/bf00274579" @default.
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