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- W343319912 abstract "This chapter discusses a study to analyze homeoviscous adaptation of cellular membranes during thermal acclimation. This study examined two membranous fractions from the same tissue of differently acclimated fish to determine whether different membrane-types isolated from the same cell respond during thermal acclimation in the same way. An important aspect of all such studies is the purity of the membrane fractions. Liver microsomes were successfully prepared from green sunfish (Lepomis cyanellus) by conventional differential centrifugation techniques, although for liver mitochondria, it was necessary to develop a discontinuous Ficoll gradient centrifugation technique to remove contaminating microsomes. The acclimation of green sunfish to 5°C, 15°C, 25°C, or 34°C resulted in large and significant partial compensations of membrane fluidity as revealed by steady state fluorescence polarization techniques using 1, 6-diphenylhexatriene as probe. By contrast, liver microsomes of 5°C, 15°C, and 25°C acclimated fish were unaffected, although microsomes of 34°C acclimated fish were partially compensated with respect to 25°C acclimated fish. The analysis of phosphoglyceride fatty acid composition tended to confirm the different properties of the two membrane fractions." @default.
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- W343319912 date "1980-01-01" @default.
- W343319912 modified "2023-09-27" @default.
- W343319912 title "THE HOMEOVISCOUS ADAPTATION OF CELLULAR MEMBRANES DURING THERMAL ACCLIMATION" @default.
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- W343319912 doi "https://doi.org/10.1016/b978-0-08-024939-1.50060-5" @default.
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