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- W34392045 abstract "The activity and stability of a specific membrane function, sulfate permease, from Penicillium duponti (PD) a thermophilic fungus capable of growth between 30 and 58/sup 0/ and from Pencilium chrysogenum (PC) a mesophial capable of growth between 4 and 33/sup 0/ were compared to determine whether such activity reflects actual or optimal growth temperature. Permease was assayed by incubating derepressed mycelia (grown in media containing 1.0 mg/l cysteic acid instead of 1.0 g/l SO/sub 4/) with radioactive sulfate, collecting mycelia at 30 sec intervals and counting the /sup 35/S incorporated into mycelial pellets. Mycelia from cells grown at 8/sup 0/ (PC only), 30/sup 0/ (PC and PD) or 50/sup 0/ (PD only) were assayed. The temperature optimum from PC cells grown at either 8 or 30/sup 0/ is 25/sup 0/, while the temperature optimum from PD cells grown at either 30 or 50/sup 0/ is 45/sup 0/. However the specific activity of the permease, the shape of the temperature optimum curve and the stability of the permease vary dramatically with the growth temperature and growth stage of the mycelia. There is an apparent ability to compensate for growth at lower temperature by either an increase in permease specific activity inmore » 30/sup 0/ grown PD cells or a broadening of the temperature optimum curve for 8/sup 0/ grown PC. Transfer of cells grown in complete media (citrate No. 3 containing 4% glucose and 1 g/l sodium sulfate) to media lacking sulfate also results in derepression for sulfate permease. The time course and maximum amount of derepression observed reflects fungal growth temperature.« less" @default.
- W34392045 created "2016-06-24" @default.
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- W34392045 date "1986-05-01" @default.
- W34392045 modified "2023-09-26" @default.
- W34392045 title "Temperature effects on sulfate permease in a thermophile and related mesophile" @default.
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