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- W34533312 abstract "This chapter explains that the foreign (Ad12 viral) DNA is integrated into the hamster genome of the Ad12-transformed cell line T637 and of its revertant TR12 in an orientation co-linear with the virion genome. There is only one or a few nucleotides altered at the termini of the integrated Ad12 genome. The insert in cell line TR12 consists of one complete Ad12 genome and an additional 3.9kb fragment of Ad12 DNA, which has been derived from the right viral end and has been flip-flopped. Foreign DNA into established eukaryotic genomes has become an important technique in experimental biology and medicine with the aim to generate transgenic cells or organisms. In spite of the generality of the application of these procedures, little attention has been focused on their consequences for the recipient cell or organism. It is often implicitly assumed that foreign DNA insertion into a genome might not have consequences other than the ones aspired to by the experimenter." @default.
- W34533312 created "2016-06-24" @default.
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- W34533312 date "2011-01-01" @default.
- W34533312 modified "2023-10-16" @default.
- W34533312 title "DNA Methylation Profiles in the 5′-Upstream Region of the Human FMR1 Promoter and in an Adenovirus Transgenome" @default.
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- W34533312 doi "https://doi.org/10.1016/b978-0-12-375709-8.00030-7" @default.
- W34533312 hasPublicationYear "2011" @default.
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