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- W346448675 abstract "Thereisnostandard immunoassay forevaluating immuneresponses tomeningococcal vaccines. We developed anenzyme-linked immunosorbent assay tomeasure total levels ofantibody toNeisseria meningitdis groupA capsular polysaccharide. Fivelaboratories measured theantibody levels insixpaired pre-and postvaccination serumsamples byusing theenzyme-linked immunosorbent assay. Methylated humanserum albumin wasusedtobindnative group Apolysaccharide tomicrotiter plate surfaces. Thebetween-laboratory coefficients ofvariation forpre- andpostvaccination sera hadranges of31to91and17to31,respectively. The meanlaboratory coefficients ofvariation forpre-andpostvaccination sera, respectively, were17and11 (Molecular Biology Laboratory, Centers forDisease Control), 12and15(Immunodiagnostic Methods Laboratory, Centers forDisease Control), 22and19(Dana-Farber Cancer Institute), 38and38(Bacterial Polysaccharide Laboratory, U.S.FoodandDrugAdministration), and11and10(Praxis Biologics, Inc.). Standardization ofthis enzyme-linked immunosorbent assay should allowinterlaboratory comparison of meningococcal vaccine immunogenicity, thusproviding alaboratory-based assessment tool forevaluating meningococcal vaccines." @default.
- W346448675 created "2016-06-24" @default.
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- W346448675 date "1992-01-01" @default.
- W346448675 modified "2023-09-26" @default.
- W346448675 title "Multicenter Comparison ofLevels ofAntibody tothe Neisseria meningitidis GroupA Capsular Polysaccharide Measured byUsinganEnzyme-Linked Immunosorbent Assay" @default.
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