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• W34767495 startingPage "267" @default.
• W34767495 abstract "Rheumatic diseases are accompanied by a progressive destruction of the cartilage layers of the joints. Although the number of patients suffering from rheumatic diseases is steadily increasing, degradation mechanisms of cartilage are not yet understood, and methods for early diagnosis are not available. Although some information on pathogenesis could be obtained from the nuclear magnetic resonance (NMR) spectra of degradation products in the supernatants of cartilage specimens incubated with degradation-causing agents, the most direct information on degradation processes would come from the native cartilage as such. To obtain highly resolved NMR spectra of cartilage, application of the recently developed high-resolution magic-angle spinning (HR-MAS) NMR technique is advisable to obtain small line-widths of individual cartilage resonances. This technique is nowadays commercially available for most NMR spectrometers and has the considerable advantage that the same pulse sequences as in high-resolution NMR can be applied. Except for a MAS spinning equipment, no solid-state NMR hardware is required. Therefore, this method can be easily implemented. Here, we describe the most important requirements that are necessary to record HR-MAS NMR spectra. The capabilities of the HR-MAS technique are discussed for the 1H and 13C NMR spectra of cartilage." @default.
• W34767495 created "2016-06-24" @default.
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• W34767495 date "2004-08-06" @default.
• W34767495 modified "2023-10-12" @default.
• W34767495 title "Evaluation of Cartilage Composition and Degradation by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance" @default.
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• W34767495 doi "https://doi.org/10.1385/1-59259-821-8:267" @default.
• W34767495 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15299220" @default.
• W34767495 hasPublicationYear "2004" @default.
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