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- W347845638 abstract "According to UL24 gene sequence of duck plague virus(DPV) isolated by the Key Laboratory of Animal Diseases and Human Health of Sichuan Province,a pair of primer was designed.The full length UL24 gene was amplified by PCR and cloned into pMD18-T vector.The positive plasmid was identified by digestion with restriction enzymes(EcoRⅠ+XhoⅠ) and sequencing.Then,the DPV UL24 gene was analyzed by bioinformatics tools.The results showed that the sequence of UL24 gene was 1 230 bp,encoding 409 amino acids and had more homologous with UL24 genes of alpha herpesvirus subfamily than with those of beta and gamma subfamily of 26 strains other Herpesviridae,and it was closest genetically with poultry alpha herpesvirus(34.7%).Phylogenetic studies demonstrated that DPV UL24 protein was more closely related to that of turkey herpesvirus.The prediction results showed that UL24 protein may be an outer membrane protein,containing a conserved domain without signal peptide,and having 5 potential N-glycosylation sites and 19 phosphorylation sites.Codon bias analysis showed that UL24 gene was biased in some codons,especially those codons encoding amino acids Ala,Apn,Glu,Phe,Leu,Arg,Thr and Val.The secondary structure prediction showed that the alpha helix,random coil and beta sheet were 46.94%,46.94% and 6.11%,respectively.The tertiary structure had not been gained by the way of Mock construction of molecular confirmation.The main antigenic epitopes of UL24 protein might be adjacent to the amino acid residues at 36-48,241-323 and 344-379." @default.
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- W347845638 date "2009-01-01" @default.
- W347845638 modified "2023-09-23" @default.
- W347845638 title "Analysis of molecular characteristics of duck plague virus UL24 gene." @default.
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