FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W348521906> ?p ?o ?g. }

• W348521906 abstract "We have investigated the hypothesis that preferential translation of the heat stress mRNAs requires the binding of a trans-acting protein factor. 32P-Labeled RNA probes covering the 5'-untranslated region (5'-UTR) of HSP70 mRNA were synthesized, and gel retardation and UV cross-linking assays performed to identify trans-acting sequence-specific RNA-binding factors. The results indicate that no HSP70 5'-UTR RNA-specific binding proteins exist. Reducing the stringency in the gel retardation binding analyses revealed several non-sequence-specific RNA-binding complexes. The proteins bind RNA more strongly than DNA, show minor preferences for specific sequences, but none binds more strongly to the HSP70 5'-UTR than to other non-heat stress RNAs. Ultraviolet cross-linking analysis identifies two principal HSP70 5'-UTR binding proteins, of approximately 50 and 70 kDa. The p50 binding activity is increased severalfold for all mRNAs in heat-stressed lysates, and its binding produces the primary gel-retarded complex. Further detailed analyses were performed to probe for any possible heat-influenced changes. RNA/protein interactions are not affected by capping. Neither the kinetics nor the salt sensitivity of protein binding is affected by heat. Binding analyses using partial or complete HSP70 5'-UTR give qualitatively similar conclusions. Binding analyses were also carried out with several 5'-UTRs to investigate whether a heat-induced repressor might be activated by heating. No normal mRNA-specific heat-induced binding changes are detected. We conclude that heat-induced alterations in RNA-binding proteins do not mediate preferential translation of heat stress mRNAs or repression of normal mRNAs." @default.
• W348521906 created "2016-06-24" @default.
• W348521906 creator A5062649197 @default.
• W348521906 creator A5085488112 @default.
• W348521906 date "1994-04-01" @default.
• W348521906 modified "2023-09-28" @default.
• W348521906 title "RNA/protein interactions in the 5'-untranslated leader of HSP70 mRNA in Drosophila lysates. Lack of evidence for specific protein binding." @default.
• W348521906 cites W1486005348 @default.
• W348521906 cites W1495304774 @default.
• W348521906 cites W1510533371 @default.
• W348521906 cites W1510897788 @default.
• W348521906 cites W1516043257 @default.
• W348521906 cites W1546570611 @default.
• W348521906 cites W1558382700 @default.
• W348521906 cites W1581245087 @default.
• W348521906 cites W1584553304 @default.
• W348521906 cites W1605112378 @default.
• W348521906 cites W1608246645 @default.
• W348521906 cites W1608907028 @default.
• W348521906 cites W1620364639 @default.
• W348521906 cites W1798612500 @default.
• W348521906 cites W1887878037 @default.
• W348521906 cites W1963550266 @default.
• W348521906 cites W1975943688 @default.
• W348521906 cites W2010346856 @default.
• W348521906 cites W2019464701 @default.
• W348521906 cites W2025763720 @default.
• W348521906 cites W2030456774 @default.
• W348521906 cites W2049422734 @default.
• W348521906 cites W2051512638 @default.
• W348521906 cites W2069030183 @default.
• W348521906 cites W2079729858 @default.
• W348521906 cites W2092954099 @default.
• W348521906 cites W2100025889 @default.
• W348521906 cites W2109664605 @default.
• W348521906 cites W2111841684 @default.
• W348521906 cites W2119006335 @default.
• W348521906 cites W2143656082 @default.
• W348521906 cites W2431087439 @default.
• W348521906 cites W284175395 @default.
• W348521906 cites W4294216491 @default.
• W348521906 doi "https://doi.org/10.1016/s0021-9258(17)34145-5" @default.
• W348521906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7511613" @default.
• W348521906 hasPublicationYear "1994" @default.
• W348521906 type Work @default.
• W348521906 sameAs 348521906 @default.
• W348521906 citedByCount "14" @default.
• W348521906 crossrefType "journal-article" @default.
• W348521906 hasAuthorship W348521906A5062649197 @default.
• W348521906 hasAuthorship W348521906A5085488112 @default.
• W348521906 hasBestOaLocation W3485219061 @default.
• W348521906 hasConcept C104317684 @default.
• W348521906 hasConcept C105580179 @default.
• W348521906 hasConcept C107824862 @default.
• W348521906 hasConcept C149364088 @default.
• W348521906 hasConcept C153911025 @default.
• W348521906 hasConcept C185592680 @default.
• W348521906 hasConcept C185622968 @default.
• W348521906 hasConcept C205260736 @default.
• W348521906 hasConcept C22667442 @default.
• W348521906 hasConcept C41282012 @default.
• W348521906 hasConcept C55493867 @default.
• W348521906 hasConcept C67705224 @default.
• W348521906 hasConcept C68991219 @default.
• W348521906 hasConcept C86803240 @default.
• W348521906 hasConcept C89604277 @default.
• W348521906 hasConceptScore W348521906C104317684 @default.
• W348521906 hasConceptScore W348521906C105580179 @default.
• W348521906 hasConceptScore W348521906C107824862 @default.
• W348521906 hasConceptScore W348521906C149364088 @default.
• W348521906 hasConceptScore W348521906C153911025 @default.
• W348521906 hasConceptScore W348521906C185592680 @default.
• W348521906 hasConceptScore W348521906C185622968 @default.
• W348521906 hasConceptScore W348521906C205260736 @default.
• W348521906 hasConceptScore W348521906C22667442 @default.
• W348521906 hasConceptScore W348521906C41282012 @default.
• W348521906 hasConceptScore W348521906C55493867 @default.
• W348521906 hasConceptScore W348521906C67705224 @default.
• W348521906 hasConceptScore W348521906C68991219 @default.
• W348521906 hasConceptScore W348521906C86803240 @default.
• W348521906 hasConceptScore W348521906C89604277 @default.
• W348521906 hasLocation W3485219061 @default.
• W348521906 hasOpenAccess W348521906 @default.
• W348521906 hasPrimaryLocation W3485219061 @default.
• W348521906 hasRelatedWork W1509637361 @default.
• W348521906 hasRelatedWork W1589258242 @default.
• W348521906 hasRelatedWork W1743048092 @default.
• W348521906 hasRelatedWork W1967767571 @default.
• W348521906 hasRelatedWork W1981607999 @default.
• W348521906 hasRelatedWork W1994778192 @default.
• W348521906 hasRelatedWork W2004066380 @default.
• W348521906 hasRelatedWork W2005441160 @default.
• W348521906 hasRelatedWork W2006022964 @default.
• W348521906 hasRelatedWork W2019451620 @default.
• W348521906 hasRelatedWork W2021838479 @default.
• W348521906 hasRelatedWork W2023491365 @default.
• W348521906 hasRelatedWork W2024419269 @default.
• W348521906 hasRelatedWork W2025817543 @default.
• W348521906 hasRelatedWork W2100426816 @default.
• W348521906 hasRelatedWork W2113536491 @default.

• next