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- W348532011 abstract "Electron microscopy shows that intact mitochondrla can be isolated from neck-muscle stored at 144 h post-mortem at 4 ~ Isolated mitochondria, all in the condcrL~:l configuration, have clearly defined outer and inner membranes, outer compartments and intracrlstal ~accs; a larger proportion ofswo!Icn ones was isolated from the 144 h than from the 120 h post-mortcm tissue. Mitochondria from 96 h tissue still retained the follo~ng ~ o of the initial ~lues for the ADP/O ratio, respiratory, contrd index (RCI) and state 3 respiratory rate observed for 0-5 h tissue: malate + pyrus~te, 100, ~ and 53; succinate, 80, 30 and 74; ascorbate + tetramethyl-p-phenylenedlamine (TMPD), 92, 88 and 72. Both the succinate and ascorbate-TMPD oxidase systems appear to have a critical storage time ofabout 70 h, whereas the malate + pyru~te s)~tem has one of about 96 h. Asharp decline of the ADPIO ratio, RCI and the state 3 rcspiratoD rate occurred after this time, but these three parameters were better preserved in the ascorbate-TMPD oxldase system. The o.~datlon o! the citric acid Q-cle inter~ncdiatcsln the neck-muscle mltochondr/a thus ~hows a higher sensitivity to post-mortcm agelng with respect tocytorhrome oxida~e activity. This is probably due to post-mortem muscle acidification. Inuoducn'on" @default.
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- W348532011 date "1971-01-01" @default.
- W348532011 modified "2023-09-26" @default.
- W348532011 title "Post-mortem Changes in Structure and Funct/on of Ox Muscle blltochondrla. 1. Electron Microscopic and Polarographlc Investigations K. S. Chcah with the tcchnlcal asslstancc of" @default.
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