FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W349790899> ?p ?o ?g. }

• W349790899 endingPage "1456" @default.
• W349790899 startingPage "1451" @default.
• W349790899 abstract "No AccessJournal of Urology1 Dec 1987The Use of A Radiorespirometric Assay for Testing the Antibiotic Sensitivity of Catheter-Associated Bacteria T.I. Ladd, D. Schmiel, J.C. Nickel, and J.W. Costerton T.I. LaddT.I. Ladd More articles by this author , D. SchmielD. Schmiel More articles by this author , J.C. NickelJ.C. Nickel More articles by this author , and J.W. CostertonJ.W. Costerton More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)43673-1AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail A 14C-radiorespirometric assay was used to show the sensitivity of fixed-film (sessile), catheter-associated and free-living (planktonic) cells of Pseudomonas aeruginosa to varying concentrations (100μg./mL to 1000μg./mL) tobramycin sulfate. This strain of P. aeruginosa has an MIC of 0.6μg./ml and an MBC of 50μg./mL when tested by conventional methods. When 14C-glutamic acid was used as a substrate in this radiorespirometric assay, it could be completed in less than one hour and planktonic samples showed a significant reduction in mineralization activity (evolution of 14CO2) within eight hours of the antibiotic challenge. These changes in respiratory activity appeared to be dose and time dependent. Within 18 hr. at 1000μg./mL, there was no significant residual respiratory activity in planktonic samples. Some residual respiratory activity was detected, however, in samples exposed to 100μg./mL for 36 hours. The mineralization activity of sessile catheter-associated bacteria was unaffected by four hr. and eight hr. exposures to 1000μg./mL of the antibiotic. A significant reduction in respiratory activity was recorded in catheter samples exposed for 18 hr. or more at each concentration examined. Unlike the planktonic samples, however, the antibiotic challenge failed to eradicate the metabolic activity of the attached bacteria. Antibiotic stressed, catheter-associated bacteria transferred to a post-exposure enrichment broth showed a limited ability to re-establish respiratory activity. This apparent recovery was limited to antibiotic exposures less than 24 hr. and was not observed in planktonic samples. The radioisotopic assay is a non-culture method which can be used to assess the antibiotic sensitivity of both planktonic bacteria and in situ biofilm populations. Clinically, it can be used to demonstrate that some adherent biofilm bacteria can survive the exposure to antibiotics that is achieved in routine chemotherapy. © 1987 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 138Issue 6December 1987Page: 1451-1456 Advertisement Copyright & Permissions© 1987 by The American Urological Association Education and Research, Inc.MetricsAuthor Information T.I. Ladd More articles by this author D. Schmiel More articles by this author J.C. Nickel More articles by this author J.W. Costerton More articles by this author Expand All Advertisement PDF DownloadLoading ..." @default.
• W349790899 created "2016-06-24" @default.
• W349790899 creator A5034811772 @default.
• W349790899 creator A5065602101 @default.
• W349790899 creator A5066732377 @default.
• W349790899 creator A5072638244 @default.
• W349790899 date "1987-12-01" @default.
• W349790899 modified "2023-09-27" @default.
• W349790899 title "The Use of A Radiorespirometric Assay for Testing the Antibiotic Sensitivity of Catheter-Associated Bacteria" @default.
• W349790899 cites W1523651747 @default.
• W349790899 cites W1534263686 @default.
• W349790899 cites W1585692779 @default.
• W349790899 cites W1595129976 @default.
• W349790899 cites W1597479086 @default.
• W349790899 cites W1897469112 @default.
• W349790899 cites W1986905078 @default.
• W349790899 cites W2017130803 @default.
• W349790899 cites W2051696359 @default.
• W349790899 cites W2059289599 @default.
• W349790899 cites W2061465060 @default.
• W349790899 cites W2075152646 @default.
• W349790899 cites W2079130094 @default.
• W349790899 cites W2079452790 @default.
• W349790899 cites W2135798250 @default.
• W349790899 cites W2172882969 @default.
• W349790899 cites W2768727877 @default.
• W349790899 doi "https://doi.org/10.1016/s0022-5347(17)43673-1" @default.
• W349790899 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2890772" @default.
• W349790899 hasPublicationYear "1987" @default.
• W349790899 type Work @default.
• W349790899 sameAs 349790899 @default.
• W349790899 citedByCount "27" @default.
• W349790899 countsByYear W3497908992013 @default.
• W349790899 crossrefType "journal-article" @default.
• W349790899 hasAuthorship W349790899A5034811772 @default.
• W349790899 hasAuthorship W349790899A5065602101 @default.
• W349790899 hasAuthorship W349790899A5066732377 @default.
• W349790899 hasAuthorship W349790899A5072638244 @default.
• W349790899 hasConcept C2775832221 @default.
• W349790899 hasConcept C2777637488 @default.
• W349790899 hasConcept C2778806431 @default.
• W349790899 hasConcept C2778974779 @default.
• W349790899 hasConcept C501593827 @default.
• W349790899 hasConcept C523546767 @default.
• W349790899 hasConcept C54355233 @default.
• W349790899 hasConcept C71924100 @default.
• W349790899 hasConcept C86803240 @default.
• W349790899 hasConcept C89423630 @default.
• W349790899 hasConceptScore W349790899C2775832221 @default.
• W349790899 hasConceptScore W349790899C2777637488 @default.
• W349790899 hasConceptScore W349790899C2778806431 @default.
• W349790899 hasConceptScore W349790899C2778974779 @default.
• W349790899 hasConceptScore W349790899C501593827 @default.
• W349790899 hasConceptScore W349790899C523546767 @default.
• W349790899 hasConceptScore W349790899C54355233 @default.
• W349790899 hasConceptScore W349790899C71924100 @default.
• W349790899 hasConceptScore W349790899C86803240 @default.
• W349790899 hasConceptScore W349790899C89423630 @default.
• W349790899 hasIssue "6" @default.
• W349790899 hasLocation W3497908991 @default.
• W349790899 hasLocation W3497908992 @default.
• W349790899 hasOpenAccess W349790899 @default.
• W349790899 hasPrimaryLocation W3497908991 @default.
• W349790899 hasRelatedWork W1968602861 @default.
• W349790899 hasRelatedWork W2042611239 @default.
• W349790899 hasRelatedWork W2090357739 @default.
• W349790899 hasRelatedWork W2112277666 @default.
• W349790899 hasRelatedWork W2115593551 @default.
• W349790899 hasRelatedWork W2119381352 @default.
• W349790899 hasRelatedWork W2123659842 @default.
• W349790899 hasRelatedWork W2159278933 @default.
• W349790899 hasRelatedWork W2731516015 @default.
• W349790899 hasRelatedWork W4285398174 @default.
• W349790899 hasVolume "138" @default.
• W349790899 isParatext "false" @default.
• W349790899 isRetracted "false" @default.
• W349790899 magId "349790899" @default.
• W349790899 workType "article" @default.