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- W355357859 abstract "This chapter discusses a study focusing on detection of herbicides using chloroplasts immobilized in polyvinyl alcohol. A biosensor immobilizing chloroplasts in a photocrosslinkable polyvinyl alcohol polymer bearing styryl pyridinium groups is developed. The immobilizing process is simple and leads to a final membrane which allows good storage stability in a dry state. The rate of immobilized chloroplasts activity is 25% after 32 days storage, whereas this rate for free choroplasts is obtained after 5 days. The optimal temperature and pH are respectively 25°C, and pH 5.5 for the immobilized chloroplasts 35°C and pH 7 for the free chloroplasts. This study shows the efficiency of the technique by the detection of the herbicides diuron and atrazine which inhibit the photosynthetic electron transfer at the level of the photosystem II plastoquinone binding protein. Two methods of detection of the photosynthetic activity are used, a Clark-type oxygen electrode or a tiny three-electrode cell with detection of the current produced. The results showed that the immobilization of the chloroplasts in polyvinyl alcohol was a useful method for the detection of herbicides." @default.
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- W355357859 date "1994-01-01" @default.
- W355357859 modified "2023-09-25" @default.
- W355357859 title "Detection of Herbicides Using Chloroplasts Immobilized in Polyvinyl Alcohol" @default.
- W355357859 doi "https://doi.org/10.1016/b978-1-85617-242-4.50265-2" @default.
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