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- W35774072 abstract "Abstract In mast cells, IL-13 protein is robustly expressed with IgE activation; IL-13 mRNA transcript also increases, but rapidly returns to baseline levels within several hours. Based on these observations, we postulated that miRNAs may regulate IL-13 protein expression at the post-transcriptional level. miRNAs play roles in a number of biologic processes but little is known about their function in mediating allergic disease. To explore if miRNAs contribute to IL-13 regulation and other IgE responses, we performed a microarray screen for miRNAs regulated by IgE in both mouse and human mast cells. We found that IgE stimulation resulted in widespread depression of miRNAs, including downregulation of the Let-7 miRNA family. We looked for proteins expressed in IgE activated mast cells that were potentially targeted by Let-7. We found a highly conserved Let-7 binding site in the 3’UTR of the IL-13 gene. We further demonstrate that the IL-13 UTR is regulated by Let-7; forced expression of Let-7 represses expression of a reporter gene with the IL-13 UTR, and repression is abrogated by mutation of the Let-7 binding site. Conversely, we show that Let-7 inhibition results in increased expression of a reporter gene with the IL-13 UTR, either by transfection of “antagomirs”, or by overexpression of the Let-7 family inhibitor, LIN28B. These results suggest that in mast cells, IL-13 protein expression is regulated by Let-7 miRNAs and support a role for miRNAs mediating IgE responses." @default.
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- W35774072 date "2010-04-01" @default.
- W35774072 modified "2023-09-25" @default.
- W35774072 title "Regulation of IL-13 in mast cells by the Let-7 miRNA family (86.15)" @default.
- W35774072 doi "https://doi.org/10.4049/jimmunol.184.supp.86.15" @default.
- W35774072 hasPublicationYear "2010" @default.
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