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- W359292317 abstract "In this paper, we report the genetic and molecular characterization of JIL-1, a chromatin associated protein kinase. JEL-l maps to the 68A4-5 region of polytene chromosomes and is very likely to be a nonessential gene in this region as suggested by a series of deficiency mapping and rescue experiments. However, JDL-l ribozyme was able to mildly affect the late embryogenesis by reducing the hatch rate compared to the control group. We also further address the possible role of JDL-l in gene transcription by double- label immunostaining of polytene chromosomes using anti-Pol IIA, anti-Pol IIO, anti- phosphorylated histone H3 at serine 10 and and-JIL-l antibodies. The JIL-1 banding pattern is distinct yet overlaps with the banding pattern of Pol HA, Pol IIO and phosphorylated histone H3 at serine 10. Most significantly, JIL-1 does not preferentially localize within the highly transcriptionally active puff; instead it binds more strongly to boundaries of puffs compared to its weak staining of the central region of puffs. Polytene chromosome staining suggests that JIL-1 may play a role in defining competent chromatin domains for transcription possibly by phosphorylation of histone H3 at residues which are different from" @default.
- W359292317 created "2016-06-24" @default.
- W359292317 creator A5026349233 @default.
- W359292317 date "2018-08-13" @default.
- W359292317 modified "2023-10-18" @default.
- W359292317 title "Molecular and genetic analysis of JIL-1, a chromatin associated protein kinase implicated in transcriptional regulation in Drosophila" @default.
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