FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W360729914> ?p ?o ?g. }

• W360729914 abstract "DNA is a polymer of nucleotides, each nucleotide consisting of a negatively charged phosphate group, a ribose sugar unit, and a purine or pyrimidine base. The secondary structure of DNA is commonly a double-helix with Watson-Crick paired bases. Several secondary structures, all with these features, are observed for DNA. Due to the negative charge of DNA, it is always accompanied by cations, referred to as counterions. The concentration and charge, as well as the specific molecular details of the counterions, have a profound impact on the secondary structure of DNA. Also the activity of water in the system, which hydrate the molecules, comes into play. The thesis looks into some of these systems and their behavior, with nuclear magnetic resonance, NMR, as the main tool of investigation. The thesis is based on the following publications and manuscripts:Papers I-III deal with the unusual transition from the B- to the C-form of fibrous LiDNA as the water activity is lowered. A multiple-quantum NMR diffusion experiment is designed, and the diffusion of Li+ and Cs+ in oriented polymeric DNA measured using NMR. It is found that the Li+ diffusion is very slow compared to that of Cs+. Molecular dynamics simulations show strong complexation of Li+ with the charged DNA phosphate oxygens O1' and O2'. The phosphate orientations of the B- and C-forms of DNA with LiDNA are measured by 31P solid-state NMR, and a new model for the C-form is built. The new model involves a high degree of BII nucleotides, which could be of relevance to the DNA-protein recognition process. Papers IV-V set to investigate the behavior of the biologically interesting polyamines with oligomeric DNA in solution and oriented polymeric DNA fibers. The diffusion, measured by 1H NMR, of some polyamines with oligonucleotides in the Z-form and B-form, show a higher degree of association to the Z-form. 1H NMR NOE measurements show the association with DNA to be weak with both DNA forms, although a slightly shorter polyamine correlation time is observed with the Z-form. The transition of fibrous DNA from the B-form to the A-form with some polyamines but not with others is investigated. 1H NMR diffusion measurements show no unusual behavior, but some characteristic 2H spectral features are observed. No direct molecular reason for the differentent impacts on DNA structure by different polyamines can be isolated, and the effect is believed to be thermodynamic in origin. Paper VI shows that DNA complexed with the suspected intercalator bipyridyl(ethylenediamine)Pt(II)2+ remains in the B-form, meaning that the molecule does not interacalate fibrous DNA to a high degree. This is seen by application of a number of methods sensitive to the molecular conformation, amongst those extraction of the phosphate orientation using 31P solid-state NMR." @default.
• W360729914 created "2016-06-24" @default.
• W360729914 creator A5061178123 @default.
• W360729914 date "2000-01-01" @default.
• W360729914 modified "2023-09-24" @default.
• W360729914 title "NMR studies of DNA structure and counterion behaviour" @default.
• W360729914 hasPublicationYear "2000" @default.
• W360729914 type Work @default.
• W360729914 sameAs 360729914 @default.
• W360729914 citedByCount "0" @default.
• W360729914 crossrefType "journal-article" @default.
• W360729914 hasAuthorship W360729914A5061178123 @default.
• W360729914 hasConcept C104317684 @default.
• W360729914 hasConcept C145148216 @default.
• W360729914 hasConcept C159467904 @default.
• W360729914 hasConcept C178790620 @default.
• W360729914 hasConcept C185592680 @default.
• W360729914 hasConcept C18903297 @default.
• W360729914 hasConcept C2778530040 @default.
• W360729914 hasConcept C2779965526 @default.
• W360729914 hasConcept C32909587 @default.
• W360729914 hasConcept C34814092 @default.
• W360729914 hasConcept C512185932 @default.
• W360729914 hasConcept C552990157 @default.
• W360729914 hasConcept C55493867 @default.
• W360729914 hasConcept C61234307 @default.
• W360729914 hasConcept C71240020 @default.
• W360729914 hasConcept C77122620 @default.
• W360729914 hasConcept C8010536 @default.
• W360729914 hasConcept C86803240 @default.
• W360729914 hasConceptScore W360729914C104317684 @default.
• W360729914 hasConceptScore W360729914C145148216 @default.
• W360729914 hasConceptScore W360729914C159467904 @default.
• W360729914 hasConceptScore W360729914C178790620 @default.
• W360729914 hasConceptScore W360729914C185592680 @default.
• W360729914 hasConceptScore W360729914C18903297 @default.
• W360729914 hasConceptScore W360729914C2778530040 @default.
• W360729914 hasConceptScore W360729914C2779965526 @default.
• W360729914 hasConceptScore W360729914C32909587 @default.
• W360729914 hasConceptScore W360729914C34814092 @default.
• W360729914 hasConceptScore W360729914C512185932 @default.
• W360729914 hasConceptScore W360729914C552990157 @default.
• W360729914 hasConceptScore W360729914C55493867 @default.
• W360729914 hasConceptScore W360729914C61234307 @default.
• W360729914 hasConceptScore W360729914C71240020 @default.
• W360729914 hasConceptScore W360729914C77122620 @default.
• W360729914 hasConceptScore W360729914C8010536 @default.
• W360729914 hasConceptScore W360729914C86803240 @default.
• W360729914 hasLocation W3607299141 @default.
• W360729914 hasOpenAccess W360729914 @default.
• W360729914 hasPrimaryLocation W3607299141 @default.
• W360729914 hasRelatedWork W1493410964 @default.
• W360729914 hasRelatedWork W1980082804 @default.
• W360729914 hasRelatedWork W2025774053 @default.
• W360729914 hasRelatedWork W2046943018 @default.
• W360729914 hasRelatedWork W2071476696 @default.
• W360729914 hasRelatedWork W2100886995 @default.
• W360729914 hasRelatedWork W2113039499 @default.
• W360729914 hasRelatedWork W2291659093 @default.
• W360729914 hasRelatedWork W2299391766 @default.
• W360729914 hasRelatedWork W2410281138 @default.
• W360729914 hasRelatedWork W2469695457 @default.
• W360729914 hasRelatedWork W2473336541 @default.
• W360729914 hasRelatedWork W2480851788 @default.
• W360729914 hasRelatedWork W300773694 @default.
• W360729914 hasRelatedWork W3011357770 @default.
• W360729914 hasRelatedWork W49149870 @default.
• W360729914 hasRelatedWork W563024087 @default.
• W360729914 hasRelatedWork W2186855148 @default.
• W360729914 hasRelatedWork W2188728055 @default.
• W360729914 hasRelatedWork W2808528364 @default.
• W360729914 isParatext "false" @default.
• W360729914 isRetracted "false" @default.
• W360729914 magId "360729914" @default.
• W360729914 workType "article" @default.