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• W36223385 abstract "IL-33 and IL-17 family cytokines (IL-17A – IL-17F) have been shown to play roles in the pathogenesis of chronic inflammatory diseases such as rheumatoid arthritis and inflammatory bowel disease. However knowledge of their role in periodontal disease pathogenesis is limited. The aim of this study was therefore to determine clinical associations between IL-33 and IL-17 family cytokines and chronic periodontitis. In addition, to begin to investigate the biological significance of these associations using in vitro model systems.97 patients with chronic periodontitis and 77 healthy volunteers were recruited in Glasgow and Newcastle. Serum, gingival crevicular fluid (GCF) and saliva were analysed for levels of IL-33 and IL-17 family cytokines by ELISA. Periodontal tissues from 17 chronic periodontitis patients and 10 healthy subjects from Glasgow were also investigated for IL-33 and IL-17 family cytokines mRNA expression by real time PCR. Immunohistochemical analysis was also performed on tissue to investigate expression of IL-33 and IL-17E at the protein level. In vitro experiments were performed using the OKF6/TERT-2 oral keratinocyte cell line and primary human gingival epithelial (PHGE) cells. The cells were stimulated with either a live Porphyromonas gingivalis monospecies biofilm or recombinant cytokines and changes in expression of cytokines, chemokines and their receptors evaluated by real-time PCR, immunocytochemical analysis or ELISA. In addition, transcriptional activity was monitored by analysis of changes in the phosphorylation (activation) of the NF-κB p65 subunit transcription factor using serum, GCF and saliva. IL-17A and IL-17A/F levels were higher in chronic periodontitis patients, but serum IL-17E was lower. IL-17A, IL-17A/F and the serum IL-17A:IL-17E ratio correlated positively with clinical parameters. IL-33, and IL-17 family cytokine (except IL-17B) gene transcripts were higher in tissue of chronic periodontitis patients. In addition, IL-33, ST2, IL-17E and IL-17RB proteins are expressed in periodontal tissues. Furthermore, IL-33 protein expression is upregulated in tissue of chronic periodontitis patients. In vitro models showed that IL-33 and its receptors (ST2 and ST2L) are expressed by oral keratinocytes (OKF6/TERT-2 cells and PHGE cells) and IL-33 expression up-regulated in response to P. gingivalis. However, IL-33 failed to induce expression of a range of inflammatory mediators and receptors in OKF6/TERT-2 cells. In vitro, IL-17E inhibited P. gingivalis monospecies biofilm and IL-17A induced expression of chemokines (IL-8 and/or CXCL5) by OKF6/TERT-2 cells at the transcriptional level by blocking the phosphorylation (activation) of the NF-κB p65 subunit.This study demonstrates clinical associations between IL-33 and IL-17 family cytokines and chronic periodontitis. The expression of IL-33 by oral keratinocytes and its up regulation upon exposure to P. gingivalis suggest it plays a role in the innate immune response to pathogens within the periodontium. However, the role of IL-33 in the periodontal inflammatory response remains to be elucidated. The negative correlations between serum levels of IL-17A and IL-17E and correlations with disease parameters, combined with their differing effects on the induction of expression of key neutrophil chemoattractants (CXCL5 and CXCL8), suggest opposing roles in periodontal immunity. Indeed, it can be hypothesised that the differential regulation of chemokine expression is due to IL-17A having pro- and IL-17E having anti-inflammatory properties. Indeed, as neutrophils play a key role in the early events associated with periodontal disease progression, the data suggests IL-17E is a rational target for therapeutic intervention." @default.
• W36223385 created "2016-06-24" @default.
• W36223385 creator A5020142441 @default.
• W36223385 date "2014-01-01" @default.
• W36223385 modified "2023-09-26" @default.
• W36223385 title "The role of IL-33 and IL-17 family cytokines in periodontal disease" @default.
• W36223385 cites W104371847 @default.
• W36223385 cites W104930202 @default.
• W36223385 cites W135405323 @default.
• W36223385 cites W1491618207 @default.
• W36223385 cites W1501732583 @default.
• W36223385 cites W1503982748 @default.
• W36223385 cites W1510730676 @default.
• W36223385 cites W1513110975 @default.
• W36223385 cites W1513992440 @default.
• W36223385 cites W1524439383 @default.
• W36223385 cites W1525531948 @default.
• W36223385 cites W1527404918 @default.
• W36223385 cites W1533630422 @default.
• W36223385 cites W1538802759 @default.
• W36223385 cites W1551727579 @default.
• W36223385 cites W1566992315 @default.
• W36223385 cites W1568265476 @default.
• W36223385 cites W1583735966 @default.
• W36223385 cites W1592394199 @default.
• W36223385 cites W1598656707 @default.
• W36223385 cites W1600596214 @default.
• W36223385 cites W1602448887 @default.
• W36223385 cites W1640605148 @default.
• W36223385 cites W1654734080 @default.
• W36223385 cites W1783384839 @default.
• W36223385 cites W1810878070 @default.
• W36223385 cites W1825388809 @default.
• W36223385 cites W1826097838 @default.
• W36223385 cites W1826204675 @default.
• W36223385 cites W1834617037 @default.
• W36223385 cites W1852953118 @default.
• W36223385 cites W1856119395 @default.
• W36223385 cites W1873474378 @default.
• W36223385 cites W1884635290 @default.
• W36223385 cites W1897323729 @default.
• W36223385 cites W1898387047 @default.
• W36223385 cites W1910581214 @default.
• W36223385 cites W1916213760 @default.
• W36223385 cites W1918766733 @default.
• W36223385 cites W1919632652 @default.
• W36223385 cites W1921587687 @default.
• W36223385 cites W1925516831 @default.
• W36223385 cites W1932041686 @default.
• W36223385 cites W1944675847 @default.
• W36223385 cites W1948195609 @default.
• W36223385 cites W1948284414 @default.
• W36223385 cites W1958473724 @default.
• W36223385 cites W1959330059 @default.
• W36223385 cites W1960878940 @default.
• W36223385 cites W1963138732 @default.
• W36223385 cites W1963818418 @default.
• W36223385 cites W1964052073 @default.
• W36223385 cites W1964370299 @default.
• W36223385 cites W1964544797 @default.
• W36223385 cites W1965427749 @default.
• W36223385 cites W1965449674 @default.
• W36223385 cites W1966225420 @default.
• W36223385 cites W1966449856 @default.
• W36223385 cites W1966755664 @default.
• W36223385 cites W1968070392 @default.
• W36223385 cites W1968268556 @default.
• W36223385 cites W1968295332 @default.
• W36223385 cites W1968955229 @default.
• W36223385 cites W1969078493 @default.
• W36223385 cites W1969598368 @default.
• W36223385 cites W1969693921 @default.
• W36223385 cites W1969787878 @default.
• W36223385 cites W1969848441 @default.
• W36223385 cites W1970278160 @default.
• W36223385 cites W1972476901 @default.
• W36223385 cites W1973874869 @default.
• W36223385 cites W1974652468 @default.
• W36223385 cites W1974986519 @default.
• W36223385 cites W1975075371 @default.
• W36223385 cites W1975891067 @default.
• W36223385 cites W1976156103 @default.
• W36223385 cites W1979267469 @default.
• W36223385 cites W1979542628 @default.
• W36223385 cites W1980339974 @default.
• W36223385 cites W1980706794 @default.
• W36223385 cites W1980899266 @default.
• W36223385 cites W1981959283 @default.
• W36223385 cites W1982381891 @default.
• W36223385 cites W1982955578 @default.
• W36223385 cites W1982966123 @default.
• W36223385 cites W1983239749 @default.
• W36223385 cites W1983260461 @default.
• W36223385 cites W1984922621 @default.
• W36223385 cites W1985043041 @default.
• W36223385 cites W1986354998 @default.
• W36223385 cites W1986364099 @default.
• W36223385 cites W1986608275 @default.
• W36223385 cites W1987582894 @default.
• W36223385 cites W1987628377 @default.

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