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- W3649600 abstract "Microtubules which make up the spindle fibers in living cells are highly dynamic. This paper first reviews some history of visualizing individual microtubules (which are only 25 nm in width) and other submicroscopic filaments with the light microscope. We then describe the asymmetric growth and shortening of freshly exposed micro-tubule ends observed with video-enhanced DIC microscopy. Plus and minus ends were exposed by cutting the microtubule with a UV microbeam. Our results indicate that a simple GTP cap is inadequate to explain the stability of a particular microtubule end. Instead, a propagating (conformational) change is likely to be involved in the rapid disassembly of microtubules whenever the cap at the plus (but not minus) end has been removed. The observations suggest a potentially important mechanism that can selectively govern the growth and shortening of individual microtubules in living cells." @default.
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- W3649600 date "1988-01-01" @default.
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- W3649600 title "Manipulating Single Microtubules" @default.
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- W3649600 doi "https://doi.org/10.1007/978-3-7091-9011-1_7" @default.
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