Matches in SemOpenAlex for { <https://semopenalex.org/work/W365803441> ?p ?o ?g. }
Showing items 1 to 58 of
58
with 100 items per page.
- W365803441 endingPage "583" @default.
- W365803441 startingPage "571" @default.
- W365803441 abstract "The reaction mechanism of p-nitrophenylphosphatase (PNNPase) of SR was elucidated by analyzing its partial reaction using radioactive PNPP as substrate. The following results were obtained: 1. Phosphoprotein was produced by the reaction of SR with PNPP at an alkaline pH and in the presence of a high concentration of Ca2+ ions, where it decomposed very slowly. the amount of phosphoprotein reached 0.2 mol/105g in the presence of 10 mM CaCl2 and 1 mM MgCl2 at pH 8.75 and 15°C. The rate of phosphoprotein formation of PNPPase was much lower than that of ATPase. The amount of phosphoprotein decreased, while the rate of PNP liberation increased, immediately after the pH and/of free Ca2+ concentration was reduced. 2. When ADP reacted with 32P-labelled phosphoprotein produced from (32P)PNPP, the amount of 32P-labelled phosphoprotein decreased rapidly and (γ-32P)ATP was formed almost stoichiometrically. The pH stability and hydroxylamine sensitivity of phosphoprotein of PNPPase denatured with TCA were the same as those of phosphoprotein of ATPase. 3. The phosphorylation reaction was started by adding 32P)PNPP and stopped by dilution with a 40-fold volume of the reaction mixture containing unlabelled PNPP, then the time course of the subsequent decrease in the amount of phosphoprotein was measured. The time course was biphasic, indicating the existence of two kinds of phosphoprotein. In the presence of 5 mM CaCl2 and 5 mM MgCl2 at pH 8.0, the decay constants were about 2 min−1 and 0.1 min−1, respectively. The ratio of the amounts of fast and slowly decomposing components of the phosphoprotein was about 1 : 4. The decay rates and the ratio of the two components were unaffected by preincubation of SR with PNP. The decay constant of the slow phase increased when the Mg2+ concentration increased or the Ca2+ concentration decreased. However, the decay constant of the rapid phase was virtually unaffected by changing concentrations of Mg2+ and Ca2+ ions. 4. The time course of PNP liberation was resolved into two phases consisting of a fast initial phase (burst) and a slow steady phase. The burst size of PNP liberation was about 0.5 mol/105, which was larger than the amount of phosphoprotein under the same conditions, i.e., 0.25 mol/105 g." @default.
- W365803441 created "2016-06-24" @default.
- W365803441 creator A5029731768 @default.
- W365803441 creator A5068438369 @default.
- W365803441 date "1978-02-01" @default.
- W365803441 modified "2023-10-15" @default.
- W365803441 title "Reaction Mechanism of p-Nitrophenylphosphatase of Sarcoplasmic Reticulum" @default.
- W365803441 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a131945" @default.
- W365803441 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24625" @default.
- W365803441 hasPublicationYear "1978" @default.
- W365803441 type Work @default.
- W365803441 sameAs 365803441 @default.
- W365803441 citedByCount "29" @default.
- W365803441 crossrefType "journal-article" @default.
- W365803441 hasAuthorship W365803441A5029731768 @default.
- W365803441 hasAuthorship W365803441A5068438369 @default.
- W365803441 hasConcept C11960822 @default.
- W365803441 hasConcept C13965031 @default.
- W365803441 hasConcept C158617107 @default.
- W365803441 hasConcept C181199279 @default.
- W365803441 hasConcept C185592680 @default.
- W365803441 hasConcept C23265538 @default.
- W365803441 hasConcept C2775999725 @default.
- W365803441 hasConcept C2777061661 @default.
- W365803441 hasConcept C43617362 @default.
- W365803441 hasConcept C55493867 @default.
- W365803441 hasConceptScore W365803441C11960822 @default.
- W365803441 hasConceptScore W365803441C13965031 @default.
- W365803441 hasConceptScore W365803441C158617107 @default.
- W365803441 hasConceptScore W365803441C181199279 @default.
- W365803441 hasConceptScore W365803441C185592680 @default.
- W365803441 hasConceptScore W365803441C23265538 @default.
- W365803441 hasConceptScore W365803441C2775999725 @default.
- W365803441 hasConceptScore W365803441C2777061661 @default.
- W365803441 hasConceptScore W365803441C43617362 @default.
- W365803441 hasConceptScore W365803441C55493867 @default.
- W365803441 hasIssue "2" @default.
- W365803441 hasLocation W3658034411 @default.
- W365803441 hasLocation W3658034412 @default.
- W365803441 hasOpenAccess W365803441 @default.
- W365803441 hasPrimaryLocation W3658034411 @default.
- W365803441 hasRelatedWork W1482313825 @default.
- W365803441 hasRelatedWork W1991960895 @default.
- W365803441 hasRelatedWork W2045854791 @default.
- W365803441 hasRelatedWork W2087084848 @default.
- W365803441 hasRelatedWork W2123347941 @default.
- W365803441 hasRelatedWork W2130759455 @default.
- W365803441 hasRelatedWork W2154981999 @default.
- W365803441 hasRelatedWork W2163594668 @default.
- W365803441 hasRelatedWork W2171328131 @default.
- W365803441 hasRelatedWork W2388902991 @default.
- W365803441 hasVolume "83" @default.
- W365803441 isParatext "false" @default.
- W365803441 isRetracted "false" @default.
- W365803441 magId "365803441" @default.
- W365803441 workType "article" @default.