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- W36636105 abstract "Electrophoresis is one of the most important biotechnologies used for DNA and protein analyses. It is an indispensable technique for human genome analysis, DNA sequencing, gene analysis, gene therapy, and molecular weight determination of proteins. The chapter discusses characteristics of gels and their application to capillary electrophoresis. Capillary electrophoresis uses a fine capillary instead of a gel plate and separate biopolymers. The capillary used is made of high purity silica with an inner diameter of 50–100 μm, an outer diameter of 150–400 μm, and a length of 10–50 μm. For capillary electrophoresis, polyacrylamide is mainly used. The chapter presents an example of DNA sequencing using capillary gel electrophoresis with polyacrylamide. Recently, high-performance gels that can replace traditional gels have been developed. By using these gels for capillary electrophoresis, analysis performance has improved dramatically. However, the study of electrophoresis gels has just begun. In the future, it is hoped that new development of gels will accelerate the application of capillary gels to fast sequencing of human genomes, high performance genome mapping, genetic screening, DNA analysis, and genetic analysis for illnesses. Such development will significantly contribute to the human genome projects." @default.
- W36636105 created "2016-06-24" @default.
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- W36636105 date "2001-01-01" @default.
- W36636105 modified "2023-09-26" @default.
- W36636105 title "Application for electrophoresis" @default.
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- W36636105 doi "https://doi.org/10.1016/b978-012394690-4/50131-6" @default.
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