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- W3670497 abstract "Abstract A simplified procedure for the preparation of 35 S‐labeled brain cerebroside sulfates has been developed. The labeled sulfatides are synthesized in vivo after intracerebral administration of inorganic 35 S‐sulfate in developing rats. The animals are sacrificed three days later and brain homogenates extracted with chloroform‐methanol. The extract is subjected to mild alkaline treatment and washed with water. The organic phase is chromatographed on triethylaminoethyl‐cellulose from which sulfatides are eluted with chloroform‐methanol containing potassium acetate. Radioactive fractions are pooled, concentrated, and potassium acetate removed by dialysis against water. Alternatively, salts are removed by passing radioactive fractions through Sephadex G‐25. After evaporating to near dryness, the radioactive cerebroside sulfates are dissolved in a small volume of chloroform‐methanol and stored at −20 C. The 35 S‐sulfatides are essentially free of lipid contaminants, and 97% of the radio‐activity corresponds with sulfatides on chromatographic analysis." @default.
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- W3670497 date "1974-11-01" @default.
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- W3670497 title "Simplified procedure for preparation of35S-labeled brain sulfatide" @default.
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- W3670497 doi "https://doi.org/10.1007/bf02532611" @default.
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