Matches in SemOpenAlex for { <https://semopenalex.org/work/W36777773> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W36777773 endingPage "38" @default.
- W36777773 startingPage "25" @default.
- W36777773 abstract "Bioactive small molecules act through modulating a yet unpredictable number of targets. It is therefore of critical importance to define the cellular target proteins of a compound as an entry point to understanding its mechanism of action. Often, this can be achieved in a direct fashion by chemical proteomics. As with any affinity chromatography, immobilization of the bait to a solid support is one of the earliest and most crucial steps in the process. Interfering with structural features that are important for identification of a target protein will be detrimental to binding affinity. Also, many molecules are sensitive to heat or to certain chemicals, such as acid or base, and might be destroyed during the process of immobilization, which therefore needs to be not only efficient, but also mild. The subsequent affinity chromatography step needs to preserve molecular and conformational integrity of both bait compound and proteins in order to result in the desired specific enrichment while ensuring a high level of compatibility with downstream analysis by mass spectrometry. Thus, the right choice of detergent, buffer, and protease inhibitors is also essential. This chapter describes a widely applicable procedure for the immobilization of small molecule drugs and for drug-affinity chromatography with subsequent protein identification by mass spectrometry." @default.
- W36777773 created "2016-06-24" @default.
- W36777773 creator A5012452779 @default.
- W36777773 creator A5053976559 @default.
- W36777773 creator A5069216326 @default.
- W36777773 date "2011-10-03" @default.
- W36777773 modified "2023-09-24" @default.
- W36777773 title "Compound Immobilization and Drug-Affinity Chromatography" @default.
- W36777773 cites W1968327387 @default.
- W36777773 cites W1982796443 @default.
- W36777773 cites W1986139301 @default.
- W36777773 cites W2003973314 @default.
- W36777773 cites W2035503247 @default.
- W36777773 cites W2051265496 @default.
- W36777773 cites W2101635775 @default.
- W36777773 cites W2314049493 @default.
- W36777773 cites W2952617100 @default.
- W36777773 doi "https://doi.org/10.1007/978-1-61779-364-6_3" @default.
- W36777773 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22065216" @default.
- W36777773 hasPublicationYear "2011" @default.
- W36777773 type Work @default.
- W36777773 sameAs 36777773 @default.
- W36777773 citedByCount "10" @default.
- W36777773 countsByYear W367777732012 @default.
- W36777773 countsByYear W367777732015 @default.
- W36777773 countsByYear W367777732016 @default.
- W36777773 countsByYear W367777732017 @default.
- W36777773 countsByYear W367777732018 @default.
- W36777773 countsByYear W367777732020 @default.
- W36777773 countsByYear W367777732022 @default.
- W36777773 crossrefType "book-chapter" @default.
- W36777773 hasAuthorship W36777773A5012452779 @default.
- W36777773 hasAuthorship W36777773A5053976559 @default.
- W36777773 hasAuthorship W36777773A5069216326 @default.
- W36777773 hasConcept C104317684 @default.
- W36777773 hasConcept C161624437 @default.
- W36777773 hasConcept C162356407 @default.
- W36777773 hasConcept C179247698 @default.
- W36777773 hasConcept C181199279 @default.
- W36777773 hasConcept C185592680 @default.
- W36777773 hasConcept C21951064 @default.
- W36777773 hasConcept C2776714187 @default.
- W36777773 hasConcept C2780035454 @default.
- W36777773 hasConcept C2780430158 @default.
- W36777773 hasConcept C43617362 @default.
- W36777773 hasConcept C46111723 @default.
- W36777773 hasConcept C55493867 @default.
- W36777773 hasConcept C70721500 @default.
- W36777773 hasConcept C74187038 @default.
- W36777773 hasConcept C86803240 @default.
- W36777773 hasConcept C98274493 @default.
- W36777773 hasConceptScore W36777773C104317684 @default.
- W36777773 hasConceptScore W36777773C161624437 @default.
- W36777773 hasConceptScore W36777773C162356407 @default.
- W36777773 hasConceptScore W36777773C179247698 @default.
- W36777773 hasConceptScore W36777773C181199279 @default.
- W36777773 hasConceptScore W36777773C185592680 @default.
- W36777773 hasConceptScore W36777773C21951064 @default.
- W36777773 hasConceptScore W36777773C2776714187 @default.
- W36777773 hasConceptScore W36777773C2780035454 @default.
- W36777773 hasConceptScore W36777773C2780430158 @default.
- W36777773 hasConceptScore W36777773C43617362 @default.
- W36777773 hasConceptScore W36777773C46111723 @default.
- W36777773 hasConceptScore W36777773C55493867 @default.
- W36777773 hasConceptScore W36777773C70721500 @default.
- W36777773 hasConceptScore W36777773C74187038 @default.
- W36777773 hasConceptScore W36777773C86803240 @default.
- W36777773 hasConceptScore W36777773C98274493 @default.
- W36777773 hasLocation W367777731 @default.
- W36777773 hasLocation W367777732 @default.
- W36777773 hasOpenAccess W36777773 @default.
- W36777773 hasPrimaryLocation W367777731 @default.
- W36777773 hasRelatedWork W2092152178 @default.
- W36777773 hasRelatedWork W2096764970 @default.
- W36777773 hasRelatedWork W2253405741 @default.
- W36777773 hasRelatedWork W2313368970 @default.
- W36777773 hasRelatedWork W2329621995 @default.
- W36777773 hasRelatedWork W2428927766 @default.
- W36777773 hasRelatedWork W2887218823 @default.
- W36777773 hasRelatedWork W2949480655 @default.
- W36777773 hasRelatedWork W3129624046 @default.
- W36777773 hasRelatedWork W631871202 @default.
- W36777773 isParatext "false" @default.
- W36777773 isRetracted "false" @default.
- W36777773 magId "36777773" @default.
- W36777773 workType "book-chapter" @default.