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- W37092675 abstract "The intricate molecular events that contribute for the non-homogenous and dynamic nature of the cell membrane occur at spatial and time scales which are not readily accessible by conventional microscopy techniques. This thesis explores the combination of photonic antenna devices and fluorescence correlation spectroscopy (FCS) to achieve simultaneously a high spatial (~10 nm) and temporal (~1 μs) resolution that allow the study of the molecular dynamics of a living cell membrane in a non-invasive way. In the first part of this work, confocal microscopy and optical antenna experiments of single fluorescent dyes diffusing in water were performed to characterize and validate the experimental approach. In the second part, preliminary results on the diffusion of lipids in living cell membranes using an antenna-in-box are reported. Due to its geometry and nanophotonic properties, the antenna-in-box provides simultaneous background suppression, volume reduction and fluorescence enhancement, which in combination with FCS seems to be an ideal tool to unravel the molecular processes that govern cell membrane organization and function." @default.
- W37092675 created "2016-06-24" @default.
- W37092675 creator A5076358730 @default.
- W37092675 date "2014-09-08" @default.
- W37092675 modified "2023-09-27" @default.
- W37092675 title "Fluorescence correlation spectroscopy on 2D optical antennas for single molecule studies in living cells" @default.
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- W37092675 hasPublicationYear "2014" @default.
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