Matches in SemOpenAlex for { <https://semopenalex.org/work/W371009825> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W371009825 endingPage "335" @default.
- W371009825 startingPage "329" @default.
- W371009825 abstract "S1 gene fragment containing receptor-binding region was amplified by several sets of primers using Over-Lap PCR. The native S1 gene was modified at A + T abundant regions; n.t.777-1683, n.t.1041-1050, n.t.1236-1248, n.t.1317-1335, n.t.1590-1605; based on the same amino acid sequences. The modified gene was cloned into a yeast expression vector pPIC9K. The resultant plasmid pPIC9K- S1 was transformed into Pichia pastoris GS 115 and the protein expression was induced with methanol. SDS-PAGE confirmed that the recombinant SI was secreted in the supernatant of induced GS 115. The protein yield reached 69 mg/l. ELISA and Western blot demonstrated that the S1 could react with the convalescent sera of people infected by SARS-CoV. Furthermore, ligand blot assay showed that the recombinant S1 could react with ACE2, the receptor of SARS-CoV. The molecular mass of expressed S1 was about 70 kDa, which was higher than that of the 30 kDa expected. PNGase F deglycosylation resulted in a protein band of 30 kDa. In conclusion, the S1 gene modification rendered the high-level expression of S1 in P. pastoris GS 115 and the protein was secreted as a biologically active form which was hyperglycosylated." @default.
- W371009825 created "2016-06-24" @default.
- W371009825 creator A5025625281 @default.
- W371009825 creator A5043859599 @default.
- W371009825 creator A5047643418 @default.
- W371009825 creator A5049871921 @default.
- W371009825 creator A5058770337 @default.
- W371009825 creator A5074707535 @default.
- W371009825 creator A5083345213 @default.
- W371009825 date "2006-12-01" @default.
- W371009825 modified "2023-09-30" @default.
- W371009825 title "Modification of SARS-CoV S1 gene render expression in Pichia pastoris" @default.
- W371009825 cites W1789264759 @default.
- W371009825 cites W1966238900 @default.
- W371009825 cites W1975490802 @default.
- W371009825 cites W1976741900 @default.
- W371009825 cites W1989202752 @default.
- W371009825 cites W1990059132 @default.
- W371009825 cites W2010234064 @default.
- W371009825 cites W2017915061 @default.
- W371009825 cites W2021101799 @default.
- W371009825 cites W2092846883 @default.
- W371009825 cites W2094349654 @default.
- W371009825 cites W2116586125 @default.
- W371009825 cites W2120357935 @default.
- W371009825 cites W2126237612 @default.
- W371009825 cites W2145944641 @default.
- W371009825 cites W2146476111 @default.
- W371009825 cites W2158922816 @default.
- W371009825 cites W2399023715 @default.
- W371009825 doi "https://doi.org/10.1007/s11262-006-0072-x" @default.
- W371009825 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7088608" @default.
- W371009825 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16991004" @default.
- W371009825 hasPublicationYear "2006" @default.
- W371009825 type Work @default.
- W371009825 sameAs 371009825 @default.
- W371009825 citedByCount "3" @default.
- W371009825 crossrefType "journal-article" @default.
- W371009825 hasAuthorship W371009825A5025625281 @default.
- W371009825 hasAuthorship W371009825A5043859599 @default.
- W371009825 hasAuthorship W371009825A5047643418 @default.
- W371009825 hasAuthorship W371009825A5049871921 @default.
- W371009825 hasAuthorship W371009825A5058770337 @default.
- W371009825 hasAuthorship W371009825A5074707535 @default.
- W371009825 hasAuthorship W371009825A5083345213 @default.
- W371009825 hasBestOaLocation W3710098251 @default.
- W371009825 hasConcept C104317684 @default.
- W371009825 hasConcept C150194340 @default.
- W371009825 hasConcept C153911025 @default.
- W371009825 hasConcept C159047783 @default.
- W371009825 hasConcept C181199279 @default.
- W371009825 hasConcept C2776415932 @default.
- W371009825 hasConcept C2779222958 @default.
- W371009825 hasConcept C2779703480 @default.
- W371009825 hasConcept C2781372317 @default.
- W371009825 hasConcept C33161422 @default.
- W371009825 hasConcept C40767141 @default.
- W371009825 hasConcept C55493867 @default.
- W371009825 hasConcept C58121356 @default.
- W371009825 hasConcept C86803240 @default.
- W371009825 hasConceptScore W371009825C104317684 @default.
- W371009825 hasConceptScore W371009825C150194340 @default.
- W371009825 hasConceptScore W371009825C153911025 @default.
- W371009825 hasConceptScore W371009825C159047783 @default.
- W371009825 hasConceptScore W371009825C181199279 @default.
- W371009825 hasConceptScore W371009825C2776415932 @default.
- W371009825 hasConceptScore W371009825C2779222958 @default.
- W371009825 hasConceptScore W371009825C2779703480 @default.
- W371009825 hasConceptScore W371009825C2781372317 @default.
- W371009825 hasConceptScore W371009825C33161422 @default.
- W371009825 hasConceptScore W371009825C40767141 @default.
- W371009825 hasConceptScore W371009825C55493867 @default.
- W371009825 hasConceptScore W371009825C58121356 @default.
- W371009825 hasConceptScore W371009825C86803240 @default.
- W371009825 hasIssue "3" @default.
- W371009825 hasLocation W3710098251 @default.
- W371009825 hasLocation W3710098252 @default.
- W371009825 hasLocation W3710098253 @default.
- W371009825 hasLocation W3710098254 @default.
- W371009825 hasOpenAccess W371009825 @default.
- W371009825 hasPrimaryLocation W3710098251 @default.
- W371009825 hasRelatedWork W2019853669 @default.
- W371009825 hasRelatedWork W2019869944 @default.
- W371009825 hasRelatedWork W2351055124 @default.
- W371009825 hasRelatedWork W2353506412 @default.
- W371009825 hasRelatedWork W2362026912 @default.
- W371009825 hasRelatedWork W2368649740 @default.
- W371009825 hasRelatedWork W2369843447 @default.
- W371009825 hasRelatedWork W2370013590 @default.
- W371009825 hasRelatedWork W2391916382 @default.
- W371009825 hasRelatedWork W2392089767 @default.
- W371009825 hasVolume "33" @default.
- W371009825 isParatext "false" @default.
- W371009825 isRetracted "false" @default.
- W371009825 magId "371009825" @default.
- W371009825 workType "article" @default.