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• W37160487 abstract "Leaching of herbicides from urban and agricultural practices into surrounding waterways and aquatic reservoirs is of growing concern from both a human and an environmental health perspective. Limitations of current chemical analytical methods have prompted a conscious focus on the development of rapid, inexpensive monitoring techniques for the detection of phytotoxic chemicals in the aquatic environment. Biorecognition system research has produced a number of functioning product solutions, including several assay techniques based on the principles of chlorophyll fluorometry.This thesis adresses the development and evaluation of a robust and effective methodology for a phytotoxidty assay, based on the instrumentation of the ToxY-PAM Dual Channel Yield Analyser (Heinz Walz GmbH, Germany). The thesis details a series of experiments designed to; investigate protocol parameters likely to impact the performance of the bioassay, select a model biomaterial component to complement the assay technology, screen the assay's ability to detect a number of priority pollutants and investigate water sample pretreatment as a means to overcoming dilution, matrix interference and sensitivity challenges.It was found that the tested sample matrix conditions of colour, light history of biomaterial and the sterility of biomaterial culture conditions all have the potential to impact the performance of the assay. The marine microalgae, Nitzschia closterium, Phaeodactylum tricornutum and Dunaliella tertiolecta and the freshwater microalgae Chlorella vulgaris displayed the greatest sensitivity to the chosen priority pollutants whilst the latter three performed exceptionally under test manipulation. Of the priority pollutants tested, nonylphenol ethoxylate and chlorpyrifos could be eliminated as potential compounds for detection by the assay. Diuron was confirmed as a compound to which the assay demonstrated a very high sensitivity whilst copper results indicated the potential, with incorporation of longer incubation periods, for the heavy metal to be detected at guideline levels.The experiment series culminated in application of the ToxY-PAM-based phytotoxicity assay in two field assessments of river, estuarine and marine environments in temperate Europe and sub-tropical Queensland, Australia. When incorporated into field studies, the assay was able to contribute an important dimension to the study by verifying chemical quantification or alerting to unidentified compounds.The outcome of the project has been a novel, cost-effective and rapid monitoring method for the detection of PSII impacting herbicides in aquatic systems. Aspects of this research are directly transferable to emerging PAM technologies for aquatic monitoring of phytotoxic compounds." @default.
• W37160487 created "2016-06-24" @default.
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• W37160487 date "2005-01-01" @default.
• W37160487 modified "2023-09-27" @default.
• W37160487 title "Development and evaluation of a novel phytotoxicity assay for the aquatic environment" @default.
• W37160487 hasPublicationYear "2005" @default.
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