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- W372155184 abstract "This chapter discusses electrochemical copolymerization of modified enzymes and mediator-substituted redox polymers. Redox polymers with nonconductive polymer backbone transfer effectively redox equivalents through electron-hopping processes between adjacent redox centers from the enzyme to the electrode surface. This approach is based on a manual deposition of a mixture of the redox polymer, the enzyme, and a suitable crosslinker, and thus not very convenient for the exclusive modification of miniaturized electrode surfaces. The development of a new generation of amperometric enzyme electrodes by copolymerization of Os-complex-substituted pyrrole derivatives or ferrocene-substituted pyrroles with pyrrole-modified enzymes, and/or mediator-modified enzymes is presented. The electron-transfer kinetic and its dependence from the flexibility and concentration of the redox mediator are optimized. This approach will open the route to reagentless enzyme electrodes with direct electrical communication between the active sites of the immobilized enzymes and the electrode surface through the conducting-polymer linked redox centers simultaneously, allowing the miniaturization due to the electrochemical deposition of the conducting redox polymers exclusively on the electrode surface." @default.
- W372155184 created "2016-06-24" @default.
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- W372155184 date "1994-01-01" @default.
- W372155184 modified "2023-09-27" @default.
- W372155184 title "Electrochemical Copolymerization of Modified Enzymes and Mediator-Substituted Redoxpolymers. Towards the Development of Reagentless and Miniaturizable Amperometric Biosensors" @default.
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- W372155184 doi "https://doi.org/10.1016/b978-1-85617-242-4.50087-2" @default.
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