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- W37456834 abstract "Publisher Summary This chapter discusses the oxidative metabolism of phagocytosing leukocytes. One difficulty in the study of phagocytotic metabolism is that the changes induced by particles are complicated by the endocytotic process, including the internalization of the plasma membrane. This can be avoided by using non-particulate reagents, which trigger metabolic changes characteristic of phagocytosis. The chapter highlights the uptake of oxygen and the release of superoxide anions by guinea pig polymorphonuclear leukocytes stimulated either by cytochalasin E or bacteria. In a study described in the chapter, the oxygen-uptake trace was obtained with a Clark-type oxygen electrode. The cytochalasin is a relatively weak stimulant of the oxygen uptake compared to bacteria, but the former caused the release of a larger amount of superoxide anions than the latter. The cytochalasins also stimulate the production of hydrogen peroxide, which can be measured continuously by the fluorescence increase resulting from the formation of a fluorescent product from homovanillic acid in the presence of horseradish peroxidase." @default.
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- W37456834 date "1981-01-01" @default.
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- W37456834 title "OXIDATIVE METABOLISM OF PHAGOCYTOSING LEUKOCYTES" @default.
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- W37456834 doi "https://doi.org/10.1016/b978-0-08-026815-6.50006-6" @default.
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